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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASFKVKPEH TGPPDLYYNE TEAAKYASNS HITAIQHEMA ERALELLALP EGKSGFLLDI

70 80 90 100 110 120
GCGTGMSSEV ILDAGHMFVG VDVSRPMLEI ARQDEDLESG DFIHQDMGLG MPFRPGSFDG

130 140 150 160 170 180
AISISAIQWL CHANASDENP RKRLLFFFQS LYGCLGRGSR AVFQFYPEND EQCDLIMGQA

190 200 210 220 230 240
HKAGFNGGLV VDFPEAAKRK KVYLVLMTGG VVQLPQALTE DGEESRTQID NAGRRFVWNS

250 260 270 280
RKNEKVAKGS KAWIEAKRQR QIKQGRDVRH ESKYSGRKRK TKF


References and documentation are available.

Number of amino acids: 283 Molecular weight: 31618.90 Theoretical pI: 7.71
Amino acid composition: 
Ala (A) 24 8.5% Arg (R) 19 6.7% Asn (N) 9 3.2% Asp (D) 16 5.7% Cys (C) 4 1.4% Gln (Q) 14 4.9% Glu (E) 21 7.4% Gly (G) 27 9.5% His (H) 8 2.8% Ile (I) 13 4.6% Leu (L) 22 7.8% Lys (K) 19 6.7% Met (M) 9 3.2% Phe (F) 15 5.3% Pro (P) 11 3.9% Ser (S) 19 6.7% Thr (T) 8 2.8% Trp (W) 3 1.1% Tyr (Y) 7 2.5% Val (V) 15 5.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 38 Atomic composition: Carbon C 1394 Hydrogen H 2185 Nitrogen N 401 Oxygen O 415 Sulfur S 13 Formula: C1394H2185N401O415S13 Total number of atoms: 4408 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 27180 Abs 0.1% (=1 g/l) 0.860, assuming all pairs of Cys residues form cystines Ext. coefficient 26930 Abs 0.1% (=1 g/l) 0.852, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 48.53 This classifies the protein as unstable. Aliphatic index: 72.08 Grand average of hydropathicity (GRAVY): -0.490