ProtParam
User-provided sequence:
10 20 30 40 50 60
MASFKVKPEH TGPPDLYYNE TEAAKYASNS HITAIQHEMA ERALELLALP EGKSGFLLDI
70 80 90 100 110 120
GCGTGMSSEV ILDAGHMFVG VDVSRPMLEI ARQDEDLESG DFIHQDMGLG MPFRPGSFDG
130 140 150 160 170 180
AISISAIQWL CHANASDENP RKRLLFFFQS LYGCLGRGSR AVFQFYPEND EQCDLIMGQA
190 200 210 220 230 240
HKAGFNGGLV VDFPEAAKRK KVYLVLMTGG VVQLPQALTE DGEESRTQID NAGRRFVWNS
250 260 270 280
RKNEKVAKGS KAWIEAKRQR QIKQGRDVRH ESKYSGRKRK TKF
References and
documentation are available.
Number of amino acids: 283
Molecular weight: 31618.90
Theoretical pI: 7.71
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1394
Hydrogen H 2185
Nitrogen N 401
Oxygen O 415
Sulfur S 13
Formula: C
1394H
2185N
401O
415S
13
Total number of atoms: 4408
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27180
Abs 0.1% (=1 g/l) 0.860, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.852, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.53
This classifies the protein as unstable.
Aliphatic index: 72.08
Grand average of hydropathicity (GRAVY): -0.490