ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASGVTVSDV CKTTYEEIKK DKKHRYVIFY IRDEKQIDVE TVADRNAEYD QFLEDIQKCG 

        70         80         90        100        110        120 
PGECRYGLFD FEYMHQCQGT SESSKKQKLF LMSWCPDTAK VKKKMLYSSS FDALKKSLVG 

       130        140 
VQKYIQATDL SEASREAVEE KLRATDRQ 

References and documentation are available.

Number of amino acids: 148

Molecular weight: 17153.48

Theoretical pI: 6.73

Amino acid composition: 
Ala (A)   9	  6.1%
Arg (R)   7	  4.7%
Asn (N)   1	  0.7%
Asp (D)  12	  8.1%
Cys (C)   5	  3.4%
Gln (Q)   9	  6.1%
Glu (E)  13	  8.8%
Gly (G)   6	  4.1%
His (H)   2	  1.4%
Ile (I)   6	  4.1%
Leu (L)   9	  6.1%
Lys (K)  18	 12.2%
Met (M)   4	  2.7%
Phe (F)   6	  4.1%
Pro (P)   2	  1.4%
Ser (S)  12	  8.1%
Thr (T)   8	  5.4%
Trp (W)   1	  0.7%
Tyr (Y)   8	  5.4%
Val (V)  10	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	       753
Hydrogen    H	      1190
Nitrogen    N	       202
Oxygen      O	       237
Sulfur      S	         9

Formula: C753H1190N202O237S9
Total number of atoms: 2391

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17670
Abs 0.1% (=1 g/l)   1.030, assuming all pairs of Cys residues form cystines


Ext. coefficient    17420
Abs 0.1% (=1 g/l)   1.016, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.15
This classifies the protein as unstable.



Aliphatic index: 65.20

Grand average of hydropathicity (GRAVY): -0.719