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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH QHLIDELQRL

70 80 90 100 110 120
ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS TLAAQSRELD SLLQQRGKEC

130 140 150 160 170 180
VQLRARWAAQ SGNDAAEVAL YERLLQLRVL PGASDVHDVR FVFGDDSRCW IEVAMHGDHV

190 200 210 220
IGNSHPALDP KSRATLEHVL TVQGDLAAFL VVARDMLLAS L


References and documentation are available.

Number of amino acids: 221 Molecular weight: 25244.30 Theoretical pI: 6.41
Amino acid composition: 
Ala (A) 27 12.2% Arg (R) 22 10.0% Asn (N) 5 2.3% Asp (D) 15 6.8% Cys (C) 2 0.9% Gln (Q) 28 12.7% Glu (E) 13 5.9% Gly (G) 8 3.6% His (H) 11 5.0% Ile (I) 5 2.3% Leu (L) 27 12.2% Lys (K) 3 1.4% Met (M) 5 2.3% Phe (F) 6 2.7% Pro (P) 3 1.4% Ser (S) 14 6.3% Thr (T) 8 3.6% Trp (W) 3 1.4% Tyr (Y) 2 0.9% Val (V) 14 6.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28 Total number of positively charged residues (Arg + Lys): 25 Atomic composition: Carbon C 1085 Hydrogen H 1740 Nitrogen N 348 Oxygen O 335 Sulfur S 7 Formula: C1085H1740N348O335S7 Total number of atoms: 3515 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 19605 Abs 0.1% (=1 g/l) 0.777, assuming all pairs of Cys residues form cystines Ext. coefficient 19480 Abs 0.1% (=1 g/l) 0.772, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 57.37 This classifies the protein as unstable. Aliphatic index: 87.06 Grand average of hydropathicity (GRAVY): -0.569