ProtParam
User-provided sequence:
10 20 30 40 50 60
MASIDAFSDL ERRMDGFQKD VAQVLARQQN HARQQLQQFQ AEMRQLHNQH QHLIDELQRL
70 80 90 100 110 120
ATQRTALQQQ IHAAQQATNT TREQWRSYHE RESELSRRQS TLAAQSRELD SLLQQRGKEC
130 140 150 160 170 180
VQLRARWAAQ SGNDAAEVAL YERLLQLRVL PGASDVHDVR FVFGDDSRCW IEVAMHGDHV
190 200 210 220
IGNSHPALDP KSRATLEHVL TVQGDLAAFL VVARDMLLAS L
References and
documentation are available.
Number of amino acids: 221
Molecular weight: 25244.30
Theoretical pI: 6.41
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1085
Hydrogen H 1740
Nitrogen N 348
Oxygen O 335
Sulfur S 7
Formula: C
1085H
1740N
348O
335S
7
Total number of atoms: 3515
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19605
Abs 0.1% (=1 g/l) 0.777, assuming all pairs of Cys residues form cystines
Ext. coefficient 19480
Abs 0.1% (=1 g/l) 0.772, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.37
This classifies the protein as unstable.
Aliphatic index: 87.06
Grand average of hydropathicity (GRAVY): -0.569