ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASINLIKSL KLYKEKIWSF DFSQGILATG STDRKIKLVS VKYDDFTLID VLDETAHKKA 

        70         80         90        100        110        120 
IRSVAWRPHT SLLAAGSFDS TVSIWAKEES ADRTFEMDLL AIIEGHENEV KGVAWSNDGY 

       130        140        150        160        170        180 
YLATCSRDKS VWIWETDESG EEYECISVLQ EHSQDVKHVI WHPSEALLAS SSYDDTVRIW 

       190        200        210        220        230        240 
KDYDDDWECV AVLNGHEGTV WSSDFDKTEG VFRLCSGSDD STVRVWKYMG DDEDDQQEWV 

       250        260        270        280        290        300 
CEAILPDVHK RQVYNVAWGF NGLIASVGAD GVLAVYEEVD GEWKVFAKRA LCHGVYEINV 

       310        320        330 
VKWLELNGKT ILATGGDDGI VNFWSLEKAA 

References and documentation are available.

Number of amino acids: 330

Molecular weight: 37274.62

Theoretical pI: 4.60

Amino acid composition: 
Ala (A)  25	  7.6%
Arg (R)  10	  3.0%
Asn (N)   9	  2.7%
Asp (D)  32	  9.7%
Cys (C)   6	  1.8%
Gln (Q)   6	  1.8%
Glu (E)  27	  8.2%
Gly (G)  22	  6.7%
His (H)   9	  2.7%
Ile (I)  19	  5.8%
Leu (L)  25	  7.6%
Lys (K)  22	  6.7%
Met (M)   3	  0.9%
Phe (F)  10	  3.0%
Pro (P)   3	  0.9%
Ser (S)  29	  8.8%
Thr (T)  15	  4.5%
Trp (W)  16	  4.8%
Tyr (Y)  11	  3.3%
Val (V)  31	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1673
Hydrogen    H	      2531
Nitrogen    N	       431
Oxygen      O	       519
Sulfur      S	         9

Formula: C1673H2531N431O519S9
Total number of atoms: 5163

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   104765
Abs 0.1% (=1 g/l)   2.811, assuming all pairs of Cys residues form cystines


Ext. coefficient   104390
Abs 0.1% (=1 g/l)   2.801, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.72
This classifies the protein as stable.



Aliphatic index: 86.82

Grand average of hydropathicity (GRAVY): -0.273