ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASISLSSST VPSLNSKESS GVSAFASRSI SAVKFQFPVR RVRTGDLKFP SLSSTTRCTP 

        70         80         90        100        110        120 
RRIEAKKQTF DSFEDLLVNS DKPVLVDYYA TWCGPCQFMV PILNEVSETL KDKIQVVKID 

       130        140        150        160        170 
TEKYPSIANK YKIEALPTFI LFKDGEPCDR FEGALTAKQL IQRIEDSLKV KP 

References and documentation are available.

Number of amino acids: 172

Molecular weight: 19250.19

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  10	  5.8%
Arg (R)   9	  5.2%
Asn (N)   4	  2.3%
Asp (D)  10	  5.8%
Cys (C)   4	  2.3%
Gln (Q)   6	  3.5%
Glu (E)  10	  5.8%
Gly (G)   5	  2.9%
His (H)   0	  0.0%
Ile (I)  11	  6.4%
Leu (L)  14	  8.1%
Lys (K)  16	  9.3%
Met (M)   2	  1.2%
Phe (F)  10	  5.8%
Pro (P)  11	  6.4%
Ser (S)  21	 12.2%
Thr (T)  11	  6.4%
Trp (W)   1	  0.6%
Tyr (Y)   4	  2.3%
Val (V)  13	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	       862
Hydrogen    H	      1384
Nitrogen    N	       226
Oxygen      O	       259
Sulfur      S	         6

Formula: C862H1384N226O259S6
Total number of atoms: 2737

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11710
Abs 0.1% (=1 g/l)   0.608, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.595, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.39
This classifies the protein as unstable.



Aliphatic index: 84.42

Grand average of hydropathicity (GRAVY): -0.238