ProtParam
User-provided sequence:
10 20 30 40 50 60
MASIVVNVHD YGTWVEDGAR VQCTFCDKRM NDFNRLKYHL GNVCKDVTPC SQVPLPIREA
70 80 90 100 110 120
FFKMVMEQRY PDRKRGRSNK GFTASETAAE AEKLAQMDVA QFFYEHGVDF SAVDSTSFKK
130 140 150 160 170 180
MMMIKTVGGE GGGQMIPDSR DLNGWMFQEA LKKVQDRVKE IKASWEITGC SILFDAWIGP
190 200 210 220 230 240
KGRDLVTFVA DCPAGAVYLK SADVSDIKTD VTALTSLVNG IVEEVGVRNV TQIIACSTSG
250 260 270 280 290 300
WVGDLGKQLA GQVFWSVSLS YCLKLMLVEI GKMYSFEDIF EKVKLLLDLI NNNPSFLYVF
310 320 330 340 350 360
RENSHKVDVS SECEFVMPYL TLEHIYWVRR AGLFASPEWK KEQGIAISSF VNDSTFWESL
370 380 390 400 410 420
DKIVGSTSSL VHGWLWFSRG SKHVAYAYHF IESIKKNVAW TFKYERQFYE PTWNVIDDVW
430 440 450 460 470 480
HNNHNPLHAA GYFLNPMAYY SDDFHTYQHV YTGLAFSLVH LVKEPHLQVK IGTQLDVYRY
490 500 510 520 530 540
GRGCFMKASQ AGQLNGVSPV NWWTQKANQY PELQNLAVKI LSQTSEGASR YKLKRSVAEK
550 560 570
LLLTEGMSHC ERKHLEELAV VHYNLQLQSC KA
References and
documentation are available.
Number of amino acids: 572
Molecular weight: 65104.47
Theoretical pI: 7.00
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2942
Hydrogen H 4498
Nitrogen N 778
Oxygen O 840
Sulfur S 28
Formula: C
2942H
4498N
778O
840S
28
Total number of atoms: 9086
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 124510
Abs 0.1% (=1 g/l) 1.912, assuming all pairs of Cys residues form cystines
Ext. coefficient 123760
Abs 0.1% (=1 g/l) 1.901, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.53
This classifies the protein as stable.
Aliphatic index: 81.92
Grand average of hydropathicity (GRAVY): -0.211