ProtParam
User-provided sequence:
10 20 30 40 50 60
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SDSPYSGGVF LVTIHFPPDY
70 80 90 100 110 120
PFKPPKVAFR TKVFHPNVNS NGSICLDILK EQWSPALTIS KVLLSICSLL TDPNPDDPLV
130 140
PEIAHMYKTD RAKYESTARS WTQKYAMG
References and
documentation are available.
Number of amino acids: 148
Molecular weight: 16510.04
Theoretical pI: 7.72
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 747
Hydrogen H 1157
Nitrogen N 191
Oxygen O 215
Sulfur S 8
Formula: C
747H
1157N
191O
215S
8
Total number of atoms: 2318
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24075
Abs 0.1% (=1 g/l) 1.458, assuming all pairs of Cys residues form cystines
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 1.451, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.30
This classifies the protein as unstable.
Aliphatic index: 75.14
Grand average of hydropathicity (GRAVY): -0.309