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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIVGP ADSPYAGGVF LVTIHFPPDY 

        70         80         90        100        110        120 
PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS KVLLSICSLL TDPNPDDPLV 

       130        140 
PEIAHMYKTD RTKYEATARS WTQKYAMG


References and documentation are available. 

Number of amino acids: 148

Molecular weight: 16474.03

Theoretical pI: 7.72


Amino acid composition: 
Ala (A)  12	  8.1%
Arg (R)   4	  2.7%
Asn (N)   4	  2.7%
Asp (D)  10	  6.8%
Cys (C)   3	  2.0%
Gln (Q)   4	  2.7%
Glu (E)   5	  3.4%
Gly (G)   6	  4.1%
His (H)   4	  2.7%
Ile (I)   9	  6.1%
Leu (L)  12	  8.1%
Lys (K)  12	  8.1%
Met (M)   4	  2.7%
Phe (F)   6	  4.1%
Pro (P)  16	 10.8%
Ser (S)  11	  7.4%
Thr (T)  10	  6.8%
Trp (W)   3	  2.0%
Tyr (Y)   5	  3.4%
Val (V)   8	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%



Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       749
Hydrogen    H	      1161
Nitrogen    N	       191
Oxygen      O	       213
Sulfur      S	         7

Formula: C749H1161N191O213S7
Total number of atoms: 2321

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24075
Abs 0.1% (=1 g/l)   1.461, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   1.454, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.85
This classifies the protein as unstable.



Aliphatic index: 79.12

Grand average of hydropathicity (GRAVY): -0.256