ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASLEDLIPT VNKLQDVMYD SGIDTLDLPI LAVVGSQSSG KSSILETLVG RDFLPRGTGI 

        70         80         90        100        110        120 
VTRRPLVLQL NNISPNSPLI EEDDNSVNPH DEVTKISGFE AGTKPLEYRG KERNHADEWG 

       130        140        150        160        170        180 
EFLHIPGKRF YDFDDIKREI ENETARIAGK DKGISKIPIN LKVFSPHVLN LTLVDLPGIT 

       190        200        210        220        230        240 
KVPIGEQPPD IEKQIKNLIL DYIATPNCLI LAVSPANVDL VNSESLKLAR EVDPQGKRTI 

       250        260        270        280        290        300 
GVITKLDLMD SGTNALDILS GKMYPLKLGF VGVVNRSQQD IQLNKTVEES LDKEEDYFRK 

       310        320        330        340        350        360 
HPVYRTISTK CGTRYLAKLL NQTLLSHIRD KLPDIKTKLN TLISQTEQEL ARYGGVGATT 

       370        380        390        400        410        420 
NESRASLVLQ LMNKFSTNFI SSIDGTSSDI NTKELCGGAR IYYIYNNVFG NSLKSIDPTS 

       430        440        450        460        470        480 
NLSVLDVRTA IRNSTGPRPT LFVPELAFDL LVKPQIKLLL EPSQRCVELV YEELMKICHK 

       490        500        510        520        530        540 
CGSAELARYP KLKSMLIEVI SELLRERLQP TRSYVESLID IHRAYINTNH PNFLSATEAM 

       550        560        570        580        590        600 
DDIMKTRRKR NQELLKSKLS QQENGQTNGI NGTSSISSNI DQDSAKNSDY DDDGIDAESK 

       610        620        630        640        650        660 
QTKDKFLNYF FGKDKKGQPV FDASDKKRSI AGDGNIEDFR NLQISDFSLG DIDDLENAEP 

       670        680        690        700        710        720 
PLTEREELEC ELIKRLIVSY FDIIREMIED QVPKAVMCLL VNYCKDSVQN RLVTKLYKET 

       730        740        750 
LFEELLVEDQ TLAQDRELCV KSLGVYKKAA TLISNIL 

References and documentation are available.

Number of amino acids: 757

Molecular weight: 84971.98

Theoretical pI: 5.35

Amino acid composition: 
Ala (A)  32	  4.2%
Arg (R)  38	  5.0%
Asn (N)  44	  5.8%
Asp (D)  57	  7.5%
Cys (C)  10	  1.3%
Gln (Q)  28	  3.7%
Glu (E)  54	  7.1%
Gly (G)  41	  5.4%
His (H)   9	  1.2%
Ile (I)  60	  7.9%
Leu (L)  91	 12.0%
Lys (K)  57	  7.5%
Met (M)  11	  1.5%
Phe (F)  22	  2.9%
Pro (P)  34	  4.5%
Ser (S)  59	  7.8%
Thr (T)  43	  5.7%
Trp (W)   1	  0.1%
Tyr (Y)  22	  2.9%
Val (V)  44	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 111
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      3753
Hydrogen    H	      6085
Nitrogen    N	      1019
Oxygen      O	      1176
Sulfur      S	        21

Formula: C3753H6085N1019O1176S21
Total number of atoms: 12054

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38905
Abs 0.1% (=1 g/l)   0.458, assuming all pairs of Cys residues form cystines


Ext. coefficient    38280
Abs 0.1% (=1 g/l)   0.451, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.58
This classifies the protein as unstable.



Aliphatic index: 98.88

Grand average of hydropathicity (GRAVY): -0.363