ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASLMLSLGS TSLLPREINK DKLKLGTSAS NPFLKAKSFS RVTMTVAVKP SRFEGITMAP 

        70         80         90        100        110        120 
PDPILGVSEA FKADTNGMKL NLGVGAYRTE ELQPYVLNVV KKAENLMLER GDNKEYLPIE 

       130        140        150        160        170        180 
GLAAFNKATA ELLFGAGHPV IKEQRVATIQ GLSGTGSLRL AAALIERYFP GAKVVISSPT 

       190        200        210        220        230        240 
WGNHKNIFND AKVPWSEYRY YDPKTIGLDF EGMIADIKEA PEGSFILLHG CAHNPTGIDP 

       250        260        270        280        290        300 
TPEQWVKIAD VIQEKNHIPF FDVAYQGFAS GSLDEDAASV RLFAERGMEF FVAQSYSKNL 

       310        320        330        340        350        360 
GLYAERIGAI NVVCSSADAA TRVKSQLKRI ARPMYSNPPV HGARIVANVV GDVTMFSEWK 

       370        380        390        400        410        420 
AEMEMMAGRI KTVRQELYDS LVSKDKSGKD WSFILKQIGM FSFTGLNKAQ SDNMTDKWHV 

       430        440        450 
YMTKDGRISL AGLSLAKCEY LADAIIDSYH NVS 

References and documentation are available.

Number of amino acids: 453

Molecular weight: 49831.25

Theoretical pI: 8.18

Amino acid composition: 
Ala (A)  45	  9.9%
Arg (R)  19	  4.2%
Asn (N)  20	  4.4%
Asp (D)  23	  5.1%
Cys (C)   3	  0.7%
Gln (Q)  11	  2.4%
Glu (E)  27	  6.0%
Gly (G)  34	  7.5%
His (H)   8	  1.8%
Ile (I)  27	  6.0%
Leu (L)  39	  8.6%
Lys (K)  33	  7.3%
Met (M)  16	  3.5%
Phe (F)  20	  4.4%
Pro (P)  21	  4.6%
Ser (S)  36	  7.9%
Thr (T)  20	  4.4%
Trp (W)   6	  1.3%
Tyr (Y)  15	  3.3%
Val (V)  30	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2234
Hydrogen    H	      3518
Nitrogen    N	       596
Oxygen      O	       656
Sulfur      S	        19

Formula: C2234H3518N596O656S19
Total number of atoms: 7023

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55475
Abs 0.1% (=1 g/l)   1.113, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   1.111, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.93
This classifies the protein as stable.



Aliphatic index: 85.96

Grand average of hydropathicity (GRAVY): -0.149