ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASLQISGSV KFEPFVGFNR IRHFRPIASL GFPRFRRRFS IGRSLLLRRS SSFSGDSRES 

        70         80         90        100        110        120 
DEERFITDAE RDGSGSVLGF QLTPPGDQQT VSTSTGEITH HEEKKEAIDQ IVMADFGVPG 

       130        140        150        160        170        180 
NRAVEEGAAE VGIPSGKAEV VNNLVFVTSE AAPYSKTGGL GDVCGSLPIA LAGRGHRVMV 

       190        200        210        220        230        240 
ISPRYLNGTA ADKNYARAKD LGIRVTVNCF GGSQEVSFYH EYRDGVDWVF VDHKSYHRPG 

       250        260        270        280        290        300 
NPYGDSKGAF GDNQFRFTLL CHAACEAPLV LPLGGFTYGE KSLFLVNDWH AGLVPILLAA 

       310        320        330        340        350        360 
KYRPYGVYKD ARSILIIHNL AHQGVEPAAT YTNLGLPSEW YGAVGWVFPT WARTHALDTG 

       370        380        390        400        410        420 
EAVNVLKGAI VTSDRIITVS QGYAWEITTV EGGYGLQDLL SSRKSVINGI TNGINVDEWN 

       430        440        450        460        470        480 
PSTDEHIPFH YSADDVSEKI KCKMALQKEL GLPIRPECPM IGFIGRLDYQ KGIDLIQTAG 

       490        500        510        520        530        540 
PDLMVDDIQF VMLGSGDPKY ESWMRSMEET YRDKFRGWVG FNVPISHRIT AGCDILLMPS 

       550        560        570        580        590        600 
RFEPCGLNQL YAMRYGTIPV VHGTGGLRDT VENFNPYAEG GAGTGTGWVF TPLSKDSMVS 

       610        620        630        640        650 
ALRLAAATYR EYKQSWEGLM RRGMTRNYSW ENAAVQYEQV FQWVFMDPPY VS 

References and documentation are available.

Number of amino acids: 652

Molecular weight: 72098.55

Theoretical pI: 6.01

Amino acid composition: 
Ala (A)  47	  7.2%
Arg (R)  41	  6.3%
Asn (N)  23	  3.5%
Asp (D)  35	  5.4%
Cys (C)   8	  1.2%
Gln (Q)  19	  2.9%
Glu (E)  38	  5.8%
Gly (G)  69	 10.6%
His (H)  16	  2.5%
Ile (I)  35	  5.4%
Leu (L)  48	  7.4%
Lys (K)  23	  3.5%
Met (M)  15	  2.3%
Phe (F)  31	  4.8%
Pro (P)  34	  5.2%
Ser (S)  47	  7.2%
Thr (T)  34	  5.2%
Trp (W)  13	  2.0%
Tyr (Y)  27	  4.1%
Val (V)  49	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      3230
Hydrogen    H	      4947
Nitrogen    N	       885
Oxygen      O	       949
Sulfur      S	        23

Formula: C3230H4947N885O949S23
Total number of atoms: 10034

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   112230
Abs 0.1% (=1 g/l)   1.557, assuming all pairs of Cys residues form cystines


Ext. coefficient   111730
Abs 0.1% (=1 g/l)   1.550, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.69
This classifies the protein as stable.



Aliphatic index: 78.65

Grand average of hydropathicity (GRAVY): -0.234