ProtParam
User-provided sequence:
10 20 30 40 50 60
MASLRLFLLC LAGLIFASEA GPGGAGESKC PLMVKVLDAV RGSPAVDVAV KVFKKTADGS
70 80 90 100 110 120
WEPFASGKTA ESGELHGLTT DEKFTEGVYR VELDTKSYWK ALGISPFHEY AEVVFTANDS
130 140
GHRHYTIAAL LSPYSYSTTA VVSNPQN
References and
documentation are available.
Number of amino acids: 147
Molecular weight: 15719.82
Theoretical pI: 5.77
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 709
Hydrogen H 1094
Nitrogen N 182
Oxygen O 214
Sulfur S 4
Formula: C
709H
1094N
182O
214S
4
Total number of atoms: 2203
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20065
Abs 0.1% (=1 g/l) 1.276, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 1.268, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.15
This classifies the protein as stable.
Aliphatic index: 82.31
Grand average of hydropathicity (GRAVY): 0.001