ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASLSGLYSS SPSLKPAKNH SFKALPAQSR DSFSFPHTSK PTNLPLTLSS ARSVARDISH 

        70         80         90        100        110        120 
ADSKKELLKD PDALWKRYLD WFYQQKELGL YLDISRVGFT DEFVAEMEPR FQAAFKAMED 

       130        140        150        160        170        180 
LEKGSIANPD EGRMVGHYWL RNSKLAPKPT LKTLIENTLD SICAFSDDII SGKIKPPSSP 

       190        200        210        220        230        240 
EGRFTQILSV GIGGSALGPQ FVAEALAPDN PPLKIRFIDN TDPAGIDHQI AQLGPELAST 

       250        260        270        280        290        300 
LVVVISKSGG TPETRNGLLE VQKAFREAGL NFAKQGVAIT QENSLLDNTA RIEGWLARFP 

       310        320        330        340        350        360 
MYDWVGGRTS IMSAVGLLPA ALQGINVREM LTGAALMDEA TRTTSIKNNP AALLAMCWYW 

       370        380        390        400        410        420 
ASNGVGSKDM VVLPYKDSLL LFSRYLQQLV MESLGKEFDL DGNTVNQGLT VYGNKGSTDQ 

       430        440        450        460        470        480 
HAYIQQLRDG VHNFFATFIE VLRDRPPGHD WELEPGVTCG DYLFGMLQGT RSALYANGRE 

       490        500        510        520        530        540 
SISVTIQEVT PTSVGAIIAL YERAVGLYAS IVNINAYHQP GVEAGKKAAA EVLALQKRVL 

       550        560        570        580        590        600 
SVLNEATCKD PVEPLTLEEI ADRCHAPEEI EMIYKIIAHM SANDRVLIAE GNCGSPRSIK 

       610 
VYLGECNVDD LYA 

References and documentation are available.

Number of amino acids: 613

Molecular weight: 67048.40

Theoretical pI: 5.47

Amino acid composition: 
Ala (A)  59	  9.6%
Arg (R)  28	  4.6%
Asn (N)  26	  4.2%
Asp (D)  34	  5.5%
Cys (C)   7	  1.1%
Gln (Q)  22	  3.6%
Glu (E)  38	  6.2%
Gly (G)  46	  7.5%
His (H)  11	  1.8%
Ile (I)  35	  5.7%
Leu (L)  66	 10.8%
Lys (K)  31	  5.1%
Met (M)  14	  2.3%
Phe (F)  21	  3.4%
Pro (P)  34	  5.5%
Ser (S)  48	  7.8%
Thr (T)  30	  4.9%
Trp (W)   8	  1.3%
Tyr (Y)  19	  3.1%
Val (V)  36	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2988
Hydrogen    H	      4712
Nitrogen    N	       806
Oxygen      O	       903
Sulfur      S	        21

Formula: C2988H4712N806O903S21
Total number of atoms: 9430

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72685
Abs 0.1% (=1 g/l)   1.084, assuming all pairs of Cys residues form cystines


Ext. coefficient    72310
Abs 0.1% (=1 g/l)   1.078, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.49
This classifies the protein as stable.



Aliphatic index: 90.91

Grand average of hydropathicity (GRAVY): -0.159