ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASMAGPDHL FNLRNHFYLG AYQAAINNSE IPNLSQEDIV ERDCLVHRAY IALGSYQLVI 

        70         80         90        100        110        120 
SEIDEAAATP LQAVKLLAMY LSSPENKEST ISSLREWLAD PTVGNNAIIR LIAGTIFMHE 

       130        140        150        160        170        180 
EDYNEALKHT HSGGTMDLHA LNVQIFIKMH RSDFAEKQLR VMQQIDEDHT LTQLASAWLN 

       190        200        210        220        230        240 
LAVGGSKIQE AYLIFQDFSE KYPMTSLILN GKAVCCMHMG NFEEAETLLL EALNKDAKDP 

       250        260        270        280        290 
ETLANLVVCS LHVGKSSSRY LNQLKLSHPE HVLVKRAASA EDNFERALQS FA 

References and documentation are available.

Number of amino acids: 292

Molecular weight: 32602.10

Theoretical pI: 5.33

Amino acid composition: 
Ala (A)  32	 11.0%
Arg (R)  10	  3.4%
Asn (N)  17	  5.8%
Asp (D)  14	  4.8%
Cys (C)   4	  1.4%
Gln (Q)  13	  4.5%
Glu (E)  23	  7.9%
Gly (G)  12	  4.1%
His (H)  13	  4.5%
Ile (I)  17	  5.8%
Leu (L)  37	 12.7%
Lys (K)  13	  4.5%
Met (M)  10	  3.4%
Phe (F)  10	  3.4%
Pro (P)   8	  2.7%
Ser (S)  23	  7.9%
Thr (T)  11	  3.8%
Trp (W)   2	  0.7%
Tyr (Y)   9	  3.1%
Val (V)  14	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1442
Hydrogen    H	      2271
Nitrogen    N	       393
Oxygen      O	       440
Sulfur      S	        14

Formula: C1442H2271N393O440S14
Total number of atoms: 4560

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   0.756, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.749, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.22
This classifies the protein as unstable.



Aliphatic index: 96.99

Grand average of hydropathicity (GRAVY): -0.132