ProtParam
User-provided sequence:
10 20 30 40 50 60
MASMATGVSF SMTSGIGEGG YDVHRVATAA RTTLKLNQKN SLSESTCMLL GMNKYRGSGA
70 80 90 100 110 120
IRTVSKAVGY GQEMSKSLRV GLICGGPSAE RGISLNSARS VLDHIQGDGI NVSCYYIDPD
130 140 150 160 170 180
LKAFAISSAQ VYSNTPSDFD FKLESLAQGF SSLSELAEHL VSAVDIVFPV IHGRFGEDGG
190 200 210 220 230 240
IQELLESHNI PFVGTGSREC FRAFDKYEAS LELKELGFMT VPNYLVQGTG VDKSEIALWF
250 260 270 280 290 300
TDNQLDLEMG KVVVKPAKAG SSIGVKVAFG VNDSIKKATE LILEGIDDRV VVEVFIEDAY
310 320 330 340 350 360
EFTAIVLDVG SGSVCHPVVL MPTEVQLQFH GIGDPKENAI FDYRRKYLPT QQVTYHTPPR
370 380 390 400 410 420
FPIHVIKSIR EEASLIFQKL GLRDFARIDG WYLAPNSNLS SPVSETLGGT KSGDIIFTDI
430 440 450 460 470 480
NLISGMEQTS FLFQQASKVG FSHSNILRTI VHRACSRFPH LDWYNYGYSQ LLQGSTTLEV
490 500 510
SEDPQKVFVI FGGDTSERQV SVMSGTNVWV NLQRYVDVS
References and
documentation are available.
Number of amino acids: 519
Molecular weight: 56809.43
Theoretical pI: 5.45
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2535
Hydrogen H 3963
Nitrogen N 675
Oxygen O 773
Sulfur S 17
Formula: C
2535H
3963N
675O
773S
17
Total number of atoms: 7963
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47705
Abs 0.1% (=1 g/l) 0.840, assuming all pairs of Cys residues form cystines
Ext. coefficient 47330
Abs 0.1% (=1 g/l) 0.833, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.36
This classifies the protein as stable.
Aliphatic index: 89.90
Grand average of hydropathicity (GRAVY): -0.045