ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASMATGVSF SMTSGIGEGG YDVHRVATAA RTTLKLNQKN SLSESTCMLL GMNKYRGSGA 

        70         80         90        100        110        120 
IRTVSKAVGY GQEMSKSLRV GLICGGPSAE RGISLNSARS VLDHIQGDGI NVSCYYIDPD 

       130        140        150        160        170        180 
LKAFAISSAQ VYSNTPSDFD FKLESLAQGF SSLSELAEHL VSAVDIVFPV IHGRFGEDGG 

       190        200        210        220        230        240 
IQELLESHNI PFVGTGSREC FRAFDKYEAS LELKELGFMT VPNYLVQGTG VDKSEIALWF 

       250        260        270        280        290        300 
TDNQLDLEMG KVVVKPAKAG SSIGVKVAFG VNDSIKKATL ILEGIDDRVV VEVFIEDAYE 

       310        320        330        340        350        360 
FTAIVLDVGS GSVCHPVVLM PTEVQLQFHG IGDPKENAIF DYRRKYLPTQ QVTYHTPPRF 

       370        380        390        400        410        420 
PIHVIKSIRE EASLIFQKLG LRDFARIDGW YLAPNSNLSS PVSETLGGTK SGDIIFTDIN 

       430        440        450        460        470        480 
LISGMKQTSF LFQQASKVGF SHSNILRTIV HRACSRFPHL DWYNYGYSQL LQGSTTLEVS 

       490        500        510        520        530        540 
EDPQKVFVIF GGDTSERQVS VMSGTNVWVN LQRYVDLNVT PCLLSPSLSN SLGASSNLDN 

       550        560        570        580        590        600 
REVWVLPYSV VLRHTAEEVL AACLEAVEPV RALFTSLLQK QVMEDLMDGF KNQSWFAGFD 

       610        620        630        640        650        660 
ITDELPRKYS LKEWIKHAKE AQATVFIAVH GGIGEDGTLQ GLLEDEGVSY TGPGVLASRT 

       670        680        690        700        710        720 
CMDKVMTSQA LSNLSEFGIH TISKDVKRTE DIMHETFPNV WDELIKKLQC LTLCVKPAKD 

       730        740        750        760        770        780 
GCSTGVARLC SSEDLAVYVQ ALKDCIPRIP PNTLSKTHGM IEMPNPTPEF LIFEPFVETD 

       790        800        810        820        830        840 
EIIVSSKAKQ QLSWKGRRRW VEMTVGVIGK HGSMHSLSPS LTVKESGDIL SLEEKFQVAY 


EIKS 

References and documentation are available.

Number of amino acids: 844

Molecular weight: 92803.92

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  49	  5.8%
Arg (R)  33	  3.9%
Asn (N)  27	  3.2%
Asp (D)  44	  5.2%
Cys (C)  14	  1.7%
Gln (Q)  32	  3.8%
Glu (E)  55	  6.5%
Gly (G)  67	  7.9%
His (H)  20	  2.4%
Ile (I)  50	  5.9%
Leu (L)  80	  9.5%
Lys (K)  46	  5.5%
Met (M)  20	  2.4%
Phe (F)  38	  4.5%
Pro (P)  34	  4.0%
Ser (S)  83	  9.8%
Thr (T)  47	  5.6%
Trp (W)  10	  1.2%
Tyr (Y)  22	  2.6%
Val (V)  73	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      4138
Hydrogen    H	      6518
Nitrogen    N	      1098
Oxygen      O	      1254
Sulfur      S	        34

Formula: C4138H6518N1098O1254S34
Total number of atoms: 13042

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88655
Abs 0.1% (=1 g/l)   0.955, assuming all pairs of Cys residues form cystines


Ext. coefficient    87780
Abs 0.1% (=1 g/l)   0.946, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.58
This classifies the protein as stable.



Aliphatic index: 90.96

Grand average of hydropathicity (GRAVY): -0.071