ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASMSFALNR FSGLSSKTTL SADFDPSSRR SFLPPTRVGL KISSAAESEP DLSVTVNGLK 

        70         80         90        100        110        120 
MPNPFVIGSG PPGTNYTVMK RAFDEGWGAV IAKTVSLDAS KVINVTPRYA RLRTGSNGSA 

       130        140        150        160        170        180 
KTDVIGWQNI ELISDRPLET MLKEFERLKK EYPDRILIAS VMEEYNKTAW EELIDRVEQT 

       190        200        210        220        230        240 
GVDALEINFS CPHGMPERRM GAAVGQDCAL LDEVCGWINA KATVPVWAKM TPNITDITEP 

       250        260        270        280        290        300 
ARVSLKSGCE GIAAINTIMS VMGIDMKTLR PEPCVEGYST PGGYSYKAVR PIALAKVMNI 

       310        320        330        340        350        360 
AKMMKSEFSE DRSLSGIGGV ETGYDAAEFI LLGSNTVQVC TGVMMHGYGH VKTLCAELKD 

       370        380        390        400        410        420 
FMKQHNFSTI EEFRGHSLQY FTTHTDLVKR QKEAVEQRKA EKRGLKSDKD WTGDGFVKET 


ESMVSN 

References and documentation are available.

Number of amino acids: 426

Molecular weight: 46846.61

Theoretical pI: 6.37

Amino acid composition: 
Ala (A)  32	  7.5%
Arg (R)  22	  5.2%
Asn (N)  16	  3.8%
Asp (D)  21	  4.9%
Cys (C)   7	  1.6%
Gln (Q)   8	  1.9%
Glu (E)  32	  7.5%
Gly (G)  34	  8.0%
His (H)   6	  1.4%
Ile (I)  23	  5.4%
Leu (L)  29	  6.8%
Lys (K)  29	  6.8%
Met (M)  19	  4.5%
Phe (F)  15	  3.5%
Pro (P)  20	  4.7%
Ser (S)  36	  8.5%
Thr (T)  30	  7.0%
Trp (W)   6	  1.4%
Tyr (Y)  10	  2.3%
Val (V)  31	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2056
Hydrogen    H	      3279
Nitrogen    N	       563
Oxygen      O	       633
Sulfur      S	        26

Formula: C2056H3279N563O633S26
Total number of atoms: 6557

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48275
Abs 0.1% (=1 g/l)   1.030, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   1.022, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.96
This classifies the protein as unstable.



Aliphatic index: 76.22

Grand average of hydropathicity (GRAVY): -0.276