ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD DQKFGVPVRS 

        70         80         90        100        110        120 
FKGHSHIVQD CTLTADGAYA LSASWDKTLR LWDVATGETY QRFVGHKSDV MSVDIDKKAS 

       130        140        150        160        170        180 
MIISGSRDKT IKVWTIKGQC LATLLGHNDW VSQVRVVPNE KADDDSVTII SAGNDKMVKA 

       190        200        210        220        230        240 
WNLNQFQIEA DFIGHNSNIN TLTASPDGTL IASAGKDGEI MLWNLAAKKA MYTLSAQDEV 

       250        260        270        280        290        300 
FSLAFSPNRY WLAAATATGI KVFSLDPQYL VDDLRPEFAG YSKAAEPHAV SLAWSADGQT 

       310 
LFAGYTDNVI RVWQVMTAN 

References and documentation are available.

Number of amino acids: 319

Molecular weight: 34805.33

Theoretical pI: 5.80

Amino acid composition: 
Ala (A)  35	 11.0%
Arg (R)  10	  3.1%
Asn (N)  15	  4.7%
Asp (D)  25	  7.8%
Cys (C)   2	  0.6%
Gln (Q)  12	  3.8%
Glu (E)   9	  2.8%
Gly (G)  22	  6.9%
His (H)   7	  2.2%
Ile (I)  16	  5.0%
Leu (L)  29	  9.1%
Lys (K)  19	  6.0%
Met (M)   7	  2.2%
Phe (F)  10	  3.1%
Pro (P)   8	  2.5%
Ser (S)  28	  8.8%
Thr (T)  23	  7.2%
Trp (W)  11	  3.4%
Tyr (Y)   7	  2.2%
Val (V)  24	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1551
Hydrogen    H	      2418
Nitrogen    N	       420
Oxygen      O	       473
Sulfur      S	         9

Formula: C1551H2418N420O473S9
Total number of atoms: 4871

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71055
Abs 0.1% (=1 g/l)   2.041, assuming all pairs of Cys residues form cystines


Ext. coefficient    70930
Abs 0.1% (=1 g/l)   2.038, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 25.36
This classifies the protein as stable.



Aliphatic index: 87.81

Grand average of hydropathicity (GRAVY): -0.131