ProtParam
User-provided sequence:
10 20 30 40 50 60
MASNSIKIFA GNSHPELAEK VARRIGLSLG KVAVVQYSNR ETSVTIGESV RDEDVFILQT
70 80 90 100 110 120
GCGSINDHLM ELLIMINACR SASARRITAI IPCFPYARQD KKDKSRAPIT ARLVANMLQT
130 140 150 160 170 180
AGCNHIITMD LHASQIQGFF NVPVDNLYAE PSVLRYIREN IDTTVNPTVI VSPDAGGAKR
190 200 210 220 230 240
ATALADRLDL DFALIHKERQ KANEVSRMVL VGDVRDKLAI LVDDMADTCG TLGLAAKTLK
250 260 270 280 290 300
DNGAKAVYAI VTHGILSGKA IKVINESALE KVIVTNTIPH DDKRSLCSKI ETIDISGVLA
310 320
ECIRRIHHGE SVSVLFSVAP A
References and
documentation are available.
Number of amino acids: 321
Molecular weight: 34779.18
Theoretical pI: 8.13
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1520
Hydrogen H 2513
Nitrogen N 439
Oxygen O 462
Sulfur S 14
Formula: C
1520H
2513N
439O
462S
14
Total number of atoms: 4948
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7825
Abs 0.1% (=1 g/l) 0.225, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.214, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.56
This classifies the protein as stable.
Aliphatic index: 106.67
Grand average of hydropathicity (GRAVY): 0.064