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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASNSIKIFA GNSHPELAEK VARRIGLSLG KVAVVQYSNR ETSVTIGESV RDEDVFILQT

70 80 90 100 110 120
GCGSINDHLM ELLIMINACR SASARRITAI IPCFPYARQD KKDKSRAPIT ARLVANMLQT

130 140 150 160 170 180
AGCNHIITMD LHASQIQGFF NVPVDNLYAE PSVLRYIREN IDTTVNPTVI VSPDAGGAKR

190 200 210 220 230 240
ATALADRLDL DFALIHKERQ KANEVSRMVL VGDVRDKLAI LVDDMADTCG TLGLAAKTLK

250 260 270 280 290 300
DNGAKAVYAI VTHGILSGKA IKVINESALE KVIVTNTIPH DDKRSLCSKI ETIDISGVLA

310 320
ECIRRIHHGE SVSVLFSVAP A


References and documentation are available.

Number of amino acids: 321 Molecular weight: 34779.18 Theoretical pI: 8.13
Amino acid composition: 
Ala (A) 35 10.9% Arg (R) 20 6.2% Asn (N) 15 4.7% Asp (D) 21 6.5% Cys (C) 7 2.2% Gln (Q) 7 2.2% Glu (E) 15 4.7% Gly (G) 18 5.6% His (H) 9 2.8% Ile (I) 31 9.7% Leu (L) 27 8.4% Lys (K) 18 5.6% Met (M) 7 2.2% Phe (F) 7 2.2% Pro (P) 10 3.1% Ser (S) 23 7.2% Thr (T) 18 5.6% Trp (W) 0 0.0% Tyr (Y) 5 1.6% Val (V) 28 8.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 36 Total number of positively charged residues (Arg + Lys): 38 Atomic composition: Carbon C 1520 Hydrogen H 2513 Nitrogen N 439 Oxygen O 462 Sulfur S 14 Formula: C1520H2513N439O462S14 Total number of atoms: 4948 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 7825 Abs 0.1% (=1 g/l) 0.225, assuming all pairs of Cys residues form cystines Ext. coefficient 7450 Abs 0.1% (=1 g/l) 0.214, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 34.56 This classifies the protein as stable. Aliphatic index: 106.67 Grand average of hydropathicity (GRAVY): 0.064