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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASPATKFLT DRVALVTAST KGIGFAIAKQ LGAAGASVVV CSRKKENVDE AVAALRLENI

70 80 90 100 110 120
DAHGTTAHVG NKSDRTKLID FTLDRFTKLD ILVSNAAVNP HYGDLMKVTD SQWDKLLDLN

130 140 150 160 170 180
VKSAFELTKE AVPHLEASGR GNVVFVSSVA GYSPMNEIGA YSVMKTTLTG LSKSLALNLA

190 200 210 220 230 240
RRNIRVNSIA PGIIQTDFSQ VLFSDESEKQ KWLSQIAQRR FGDPDECAEA VAFLVSDEAS

250
YISGETIGIN GGMHARI


References and documentation are available.

Number of amino acids: 257 Molecular weight: 27617.43 Theoretical pI: 6.53
Amino acid composition: 
Ala (A) 30 11.7% Arg (R) 12 4.7% Asn (N) 12 4.7% Asp (D) 16 6.2% Cys (C) 2 0.8% Gln (Q) 7 2.7% Glu (E) 13 5.1% Gly (G) 18 7.0% His (H) 5 1.9% Ile (I) 15 5.8% Leu (L) 23 8.9% Lys (K) 16 6.2% Met (M) 5 1.9% Phe (F) 10 3.9% Pro (P) 6 2.3% Ser (S) 23 8.9% Thr (T) 16 6.2% Trp (W) 2 0.8% Tyr (Y) 4 1.6% Val (V) 22 8.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 29 Total number of positively charged residues (Arg + Lys): 28 Atomic composition: Carbon C 1216 Hydrogen H 1962 Nitrogen N 340 Oxygen O 378 Sulfur S 7 Formula: C1216H1962N340O378S7 Total number of atoms: 3903 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17085 Abs 0.1% (=1 g/l) 0.619, assuming all pairs of Cys residues form cystines Ext. coefficient 16960 Abs 0.1% (=1 g/l) 0.614, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 29.72 This classifies the protein as stable. Aliphatic index: 94.16 Grand average of hydropathicity (GRAVY): -0.039