ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASQATTLRG YNIRKRDNVF EPKSSENLNS LNQSEEEGHI GRWPPLGYEA VSAEQKSAVQ 

        70         80         90        100        110        120 
LRESQAGASI SNNMNFKAND KSFSTSTAGR MSPDTNSLHH ILPKNQVKNN GQTMDANCNN 

       130        140        150        160        170        180 
NVSNDANVPV CKNCLTSTTP LWRRDEHGAM LCNACGLFLK LHGKPRPISL KTDVIKSRNR 

       190        200        210        220        230        240 
KSNTNHAHNL DNFRNQTLIA ELKGDCNIES SGRKANRVTS EDKKKKSSQL LMGTSSTAKI 

       250        260        270        280        290        300 
SKKPKTESKE RSDSHLSATK LEVLMSGDCS RPNLKPKLPK QDTAIYQEKL LTFPSYTDVK 

       310        320        330        340        350        360 
EYSNSAHQSA FIKERSQFNA ASFPLNASHS VTSKTGADSP QLPHLSMLLG SLSSTSISNN 

       370        380        390        400        410        420 
GSEIVSNCNN GIASTAATLA PTSSRTTDSN PSEVPNQIRS TMSSPDIISA KRNDPAPLSF 

       430        440        450        460        470        480 
HMASINDMLE TRDRAISNVK TETTPPHFIP FLQSSKAPCI SKANSQSISN SVSSSDVSGR 

       490        500        510        520        530        540 
KFENHPAKDL GDQLSTKLHK EEEIIKLKTR INELELVTDL YRRHINELDG KCRALEERLQ 

       550 
RTVKQEGNKG G 

References and documentation are available.

Number of amino acids: 551

Molecular weight: 60519.70

Theoretical pI: 9.34

Amino acid composition: 
Ala (A)  37	  6.7%
Arg (R)  29	  5.3%
Asn (N)  49	  8.9%
Asp (D)  25	  4.5%
Cys (C)  10	  1.8%
Gln (Q)  20	  3.6%
Glu (E)  32	  5.8%
Gly (G)  24	  4.4%
His (H)  16	  2.9%
Ile (I)  26	  4.7%
Leu (L)  46	  8.3%
Lys (K)  45	  8.2%
Met (M)  11	  2.0%
Phe (F)  13	  2.4%
Pro (P)  28	  5.1%
Ser (S)  76	 13.8%
Thr (T)  37	  6.7%
Trp (W)   2	  0.4%
Tyr (Y)   6	  1.1%
Val (V)  19	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      2576
Hydrogen    H	      4200
Nitrogen    N	       786
Oxygen      O	       854
Sulfur      S	        21

Formula: C2576H4200N786O854S21
Total number of atoms: 8437

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20565
Abs 0.1% (=1 g/l)   0.340, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.329, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.77
This classifies the protein as unstable.



Aliphatic index: 67.68

Grand average of hydropathicity (GRAVY): -0.777