ProtParam
User-provided sequence:
10 20 30 40 50 60
MASQFLRSTN SRYVAGVLAA LGAGSAVYTL TPQQLQAKSE VDAATIKKIE EAYAKLNGPE
70 80 90 100 110 120
GAKCKSLLKK HLTKDVVEKL KTKRTKLGAT LYDCIRSGVY NLDAGVGVYA PDAEAYTLFA
130 140 150 160 170 180
PLFDKIIEDY HGFSPKQKQP PVDLGEGKTK EFPPLDPKGK YIKSTRIRCG RSLKGYPFNP
190 200 210 220 230 240
CLTQDNYLEM EGKVKKAFSE YSDKELKGKY YPLDGMGKDT QKQLIADHFL FKEGDRHLQY
250 260 270 280 290 300
ANACNFWPKG RGIFHNNDKT FLIWVNEEDH MRIISMQEGS DVGAVLDRLI KGVRGIEKQV
310 320 330 340 350 360
PFSRDDRLGW LTFCPTNLGS TVRASVHIAL PKLAARKDFI EICEKLNLQV RGIHGEHSES
370 380 390
VGGVYDISNK ARLGLSEYQA VKQMYDGVKK LIEMEEKQK
References and
documentation are available.
Number of amino acids: 399
Molecular weight: 44742.42
Theoretical pI: 9.11
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2004
Hydrogen H 3177
Nitrogen N 547
Oxygen O 585
Sulfur S 14
Formula: C
2004H
3177N
547O
585S
14
Total number of atoms: 6327
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43695
Abs 0.1% (=1 g/l) 0.977, assuming all pairs of Cys residues form cystines
Ext. coefficient 43320
Abs 0.1% (=1 g/l) 0.968, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.10
This classifies the protein as stable.
Aliphatic index: 80.68
Grand average of hydropathicity (GRAVY): -0.495