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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASQFLRSTN SRYVAGVLAA LGAGSAVYTL TPQQLQAKSE VDAATIKKIE EAYAKLNGPE

70 80 90 100 110 120
GAKCKSLLKK HLTKDVVEKL KTKRTKLGAT LYDCIRSGVY NLDAGVGVYA PDAEAYTLFA

130 140 150 160 170 180
PLFDKIIEDY HGFSPKQKQP PVDLGEGKTK EFPPLDPKGK YIKSTRIRCG RSLKGYPFNP

190 200 210 220 230 240
CLTQDNYLEM EGKVKKAFSE YSDKELKGKY YPLDGMGKDT QKQLIADHFL FKEGDRHLQY

250 260 270 280 290 300
ANACNFWPKG RGIFHNNDKT FLIWVNEEDH MRIISMQEGS DVGAVLDRLI KGVRGIEKQV

310 320 330 340 350 360
PFSRDDRLGW LTFCPTNLGS TVRASVHIAL PKLAARKDFI EICEKLNLQV RGIHGEHSES

370 380 390
VGGVYDISNK ARLGLSEYQA VKQMYDGVKK LIEMEEKQK


References and documentation are available.

Number of amino acids: 399 Molecular weight: 44742.42 Theoretical pI: 9.11
Amino acid composition: 
Ala (A) 29 7.3% Arg (R) 18 4.5% Asn (N) 13 3.3% Asp (D) 24 6.0% Cys (C) 7 1.8% Gln (Q) 16 4.0% Glu (E) 26 6.5% Gly (G) 34 8.5% His (H) 9 2.3% Ile (I) 20 5.0% Leu (L) 38 9.5% Lys (K) 44 11.0% Met (M) 7 1.8% Phe (F) 15 3.8% Pro (P) 17 4.3% Ser (S) 21 5.3% Thr (T) 17 4.3% Trp (W) 3 0.8% Tyr (Y) 18 4.5% Val (V) 23 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 50 Total number of positively charged residues (Arg + Lys): 62 Atomic composition: Carbon C 2004 Hydrogen H 3177 Nitrogen N 547 Oxygen O 585 Sulfur S 14 Formula: C2004H3177N547O585S14 Total number of atoms: 6327 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43695 Abs 0.1% (=1 g/l) 0.977, assuming all pairs of Cys residues form cystines Ext. coefficient 43320 Abs 0.1% (=1 g/l) 0.968, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.10 This classifies the protein as stable. Aliphatic index: 80.68 Grand average of hydropathicity (GRAVY): -0.495