ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASRRVLSSL LRSSSGRSAA KLVNRNPRLP SPSPARHAAP CSYLLGRVAE YATSSPASSA 

        70         80         90        100        110        120 
APSSAPAKDE GKKTYDYGGK GAIGRVCQVI GAIVDVRFED QEGLPPIMTS LEVQDHPTRL 

       130        140        150        160        170        180 
VLEVSHHLGQ NVVRTIAMDG TEGLVRGRKV LNTGAPITVP VGRATLGRIM NVLGEPIDER 

       190        200        210        220        230        240 
GEIKTEHYLP IHRDAPALVD LATGQEILAT GIKVVDLLAP YQRGGKIGLF GGAGVGKTVL 

       250        260        270        280        290        300 
IMELINNVAK AHGGFSVFAG VGERTREGND LYREMIESGV IKLGEKQSES KCALVYGQMN 

       310        320        330        340        350        360 
EPPGARARVG LTGLTVAEYF RDAEGQDVLL FIDNIFRFTQ ANSEVSALLG RIPSAVGYQP 

       370        380        390        400        410        420 
TLASDLGALQ ERITTTKKGS ITSVQAIYVP ADDLTDPAPA TTFAHLDATT VLSRQISELG 

       430        440        450        460        470        480 
IYPAVDPLDS TSRMLSPHIL GEEHYNTARG VQKVLQNYKN LQDIIAILGM DELSEDDKLT 

       490        500        510        520        530        540 
VARARKIQRF LSQPFHVAEI FTGAPGKYVD LKENINSFQG LLDGKYDDLS EQSFYMVGGI 

       550 
DEVVAKAEKI AKESAA 

References and documentation are available.

Number of amino acids: 556

Molecular weight: 59713.02

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  55	  9.9%
Arg (R)  34	  6.1%
Asn (N)  16	  2.9%
Asp (D)  30	  5.4%
Cys (C)   3	  0.5%
Gln (Q)  21	  3.8%
Glu (E)  35	  6.3%
Gly (G)  53	  9.5%
His (H)  11	  2.0%
Ile (I)  34	  6.1%
Leu (L)  54	  9.7%
Lys (K)  26	  4.7%
Met (M)  10	  1.8%
Phe (F)  14	  2.5%
Pro (P)  29	  5.2%
Ser (S)  39	  7.0%
Thr (T)  31	  5.6%
Trp (W)   0	  0.0%
Tyr (Y)  17	  3.1%
Val (V)  44	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2633
Hydrogen    H	      4255
Nitrogen    N	       743
Oxygen      O	       811
Sulfur      S	        13

Formula: C2633H4255N743O811S13
Total number of atoms: 8455

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25455
Abs 0.1% (=1 g/l)   0.426, assuming all pairs of Cys residues form cystines


Ext. coefficient    25330
Abs 0.1% (=1 g/l)   0.424, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.99
This classifies the protein as unstable.



Aliphatic index: 94.57

Grand average of hydropathicity (GRAVY): -0.147