ProtParam
User-provided sequence:
10 20 30 40 50 60
MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL
70 80 90 100 110 120
RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL
130 140 150 160 170 180
RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL AFDLDGTGKS
190 200 210 220
QSGSNSGNNS KKRKNKSSGS SMATPTHSDS HEDMKRRRLE
References and
documentation are available.
Number of amino acids: 220
Molecular weight: 24857.33
Theoretical pI: 5.94
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1072
Hydrogen H 1666
Nitrogen N 314
Oxygen O 355
Sulfur S 7
Formula: C
1072H
1666N
314O
355S
7
Total number of atoms: 3414
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.982, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.982, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.34
This classifies the protein as unstable.
Aliphatic index: 55.00
Grand average of hydropathicity (GRAVY): -1.001