ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSFNEETA KKLLTQVEFY FSDSNLPTDG FLNREVTKSK DGLVSLPLVC SFSRMRNLLG 

        70         80         90        100        110        120 
LGNINREDIP PRIVEEVANL LRTSDFLKVS NNGQRIGRGT KLSKPEEVLE QVHRRTLAAS 

       130        140        150        160        170        180 
PFEYSIKMED VSSFFSQYAK VNSVRLPPNI ADKRRFCGTA LVEFSSEQDT QDILRQSLVY 

       190        200        210        220        230        240 
AGADLVLIPK SDFDCQRENM IKQLGKSESH NEFRRGQIVK FALKWIASEE KVTNKEKPSA 

       250        260        270        280        290        300 
LKNKIKEKED KETGIADREK ENGDNSCASL CKDNTDQLVV PPWNNSNSVS SEVLKDLFQR 

       310        320        330        340        350        360 
FGSVEHIEYS GGLDSGYVWF TDSETAMKAR AAVEFVGGLV VKNNFSVALE AINGEMEREL 

       370        380        390 
WKRLSSAELE GGKEGHKKEK GKDECFENVQ PTKKARKEP 

References and documentation are available.

Number of amino acids: 399

Molecular weight: 44925.71

Theoretical pI: 6.25

Amino acid composition: 
Ala (A)  23	  5.8%
Arg (R)  23	  5.8%
Asn (N)  25	  6.3%
Asp (D)  21	  5.3%
Cys (C)   6	  1.5%
Gln (Q)  13	  3.3%
Glu (E)  41	 10.3%
Gly (G)  24	  6.0%
His (H)   4	  1.0%
Ile (I)  15	  3.8%
Leu (L)  35	  8.8%
Lys (K)  37	  9.3%
Met (M)   6	  1.5%
Phe (F)  20	  5.0%
Pro (P)  14	  3.5%
Ser (S)  38	  9.5%
Thr (T)  15	  3.8%
Trp (W)   4	  1.0%
Tyr (Y)   6	  1.5%
Val (V)  29	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      1970
Hydrogen    H	      3145
Nitrogen    N	       555
Oxygen      O	       621
Sulfur      S	        12

Formula: C1970H3145N555O621S12
Total number of atoms: 6303

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31315
Abs 0.1% (=1 g/l)   0.697, assuming all pairs of Cys residues form cystines


Ext. coefficient    30940
Abs 0.1% (=1 g/l)   0.689, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.52
This classifies the protein as unstable.



Aliphatic index: 75.71

Grand average of hydropathicity (GRAVY): -0.624