ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSSCFLRS ILFSSPTNLR SNHHLPTFFP KNYLICSHST SSRFESLSVS SIGTGSTKKS 

        70         80         90        100        110        120 
SDTRRKVKSM ATTNIGKEEK KRVEIYDLEE NLVIDLAKFT ADLSDKFCKE RGAFTVVVSG 

       130        140        150        160        170        180 
GSLIKSLRKL VESPYVDSID WARWHFFWVD ERVVPKNHDD SNYKLAYDSF LSKVPIPPGN 

       190        200        210        220        230        240 
VYAINEALSA EAAADDYETC LKHLVNTNIL RVSESTGFPK FDLMLLGMGP DGHVASLFPG 

       250        260        270        280        290        300 
HGLCNESKKW VVSISDSPKP PSERITFTFP VINSSAHVAL VVCGSGKAEA VEAALKKTGN 

       310        320 
VPPAGSVSAE DELVWFLDKP ASSKL 

References and documentation are available.

Number of amino acids: 325

Molecular weight: 35644.56

Theoretical pI: 7.64

Amino acid composition: 
Ala (A)  23	  7.1%
Arg (R)  12	  3.7%
Asn (N)  14	  4.3%
Asp (D)  18	  5.5%
Cys (C)   6	  1.8%
Gln (Q)   0	  0.0%
Glu (E)  19	  5.8%
Gly (G)  17	  5.2%
His (H)   9	  2.8%
Ile (I)  14	  4.3%
Leu (L)  29	  8.9%
Lys (K)  26	  8.0%
Met (M)   4	  1.2%
Phe (F)  17	  5.2%
Pro (P)  18	  5.5%
Ser (S)  44	 13.5%
Thr (T)  16	  4.9%
Trp (W)   5	  1.5%
Tyr (Y)   7	  2.2%
Val (V)  27	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1596
Hydrogen    H	      2500
Nitrogen    N	       424
Oxygen      O	       481
Sulfur      S	        10

Formula: C1596H2500N424O481S10
Total number of atoms: 5011

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38305
Abs 0.1% (=1 g/l)   1.075, assuming all pairs of Cys residues form cystines


Ext. coefficient    37930
Abs 0.1% (=1 g/l)   1.064, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.02
This classifies the protein as unstable.



Aliphatic index: 82.77

Grand average of hydropathicity (GRAVY): -0.185