ProtParam
User-provided sequence:
10 20 30 40 50 60
MASSSKALIE SIGSLDKDSY VSLLSKLIGE SKFVQNNPPE LIPQEDLIVK HVLDSLRPYS
70 80 90 100 110 120
TETGGGPLVI NHVAYHSGRG NLIVEYPGSV PGKILSFVGM HMDVVTANPD DWEFDPFSLS
130 140 150 160 170 180
IDGDKLRGRG TTDCLGHVAL VTELMKKLGQ AKPALKSTVV AVFIASEENS SIPGVGVDML
190 200 210 220 230 240
VKDKLLDKLK SGPLYWIDTA DKQPCVGTGG MIPWKLQFTG KLFHSGLAHK AINAMELAME
250 260 270 280 290 300
GLKEIQARFY RDFPPHPQEE VYGFATPSTM KPTQWCYPAG GINQIPGECT VSGDVRLTPF
310 320 330 340 350 360
YDVKEVITKL QEYVDDINGN IERLETRGPV SKYVLPDENL RGRLTLSFDE ASAGVACNLD
370 380 390 400 410 420
SPGFHVLCKA TEEVVGHVKP YSITGTLPLI RDLQDEGFDV QTSGYGLMAT YHAKNEYCLL
430 440
TDMCQGFDVF IRIISQLEQV
References and
documentation are available.
Number of amino acids: 440
Molecular weight: 48217.17
Theoretical pI: 5.16
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2159
Hydrogen H 3390
Nitrogen N 560
Oxygen O 651
Sulfur S 19
Formula: C
2159H
3390N
560O
651S
19
Total number of atoms: 6779
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44850
Abs 0.1% (=1 g/l) 0.930, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 0.920, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.68
This classifies the protein as stable.
Aliphatic index: 91.00
Grand average of hydropathicity (GRAVY): -0.125