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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSSSLSLS FPSSSLSRTW DYDVFPSFHG EDVRKAFLSQ ILKKEFGRKA INFFVDHEME

70 80 90 100 110 120
RGEFIGPVLK QAIKGSKIAL VLLSKNYASS WCCLDELVEI MKKESGQTVI TIFYEVDPNE

130 140 150 160 170
VKKQTGDFGK AFKETCQGKT EEKVQTWRKA LEGVGLATIA GFHSSNWFVF FI


References and documentation are available.

Number of amino acids: 172 Molecular weight: 19412.26 Theoretical pI: 7.69
Amino acid composition: 
Ala (A) 10 5.8% Arg (R) 5 2.9% Asn (N) 4 2.3% Asp (D) 7 4.1% Cys (C) 3 1.7% Gln (Q) 6 3.5% Glu (E) 14 8.1% Gly (G) 12 7.0% His (H) 3 1.7% Ile (I) 10 5.8% Leu (L) 13 7.6% Lys (K) 17 9.9% Met (M) 3 1.7% Phe (F) 15 8.7% Pro (P) 4 2.3% Ser (S) 19 11.0% Thr (T) 8 4.7% Trp (W) 4 2.3% Tyr (Y) 3 1.7% Val (V) 12 7.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 21 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 885 Hydrogen H 1362 Nitrogen N 224 Oxygen O 255 Sulfur S 6 Formula: C885H1362N224O255S6 Total number of atoms: 2732 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 26595 Abs 0.1% (=1 g/l) 1.370, assuming all pairs of Cys residues form cystines Ext. coefficient 26470 Abs 0.1% (=1 g/l) 1.364, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.11 This classifies the protein as stable. Aliphatic index: 78.20 Grand average of hydropathicity (GRAVY): -0.165