ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSSSPPAA MEVGESTNWT ELPPELTSAI LHRLGAIEIL ENAQKVCRSW RRVCKDPSMW 

        70         80         90        100        110        120 
RKIDMHNLGD LDDMDYNLEI MCRHAVDRSQ GGLVDIGIWY FGTVDLLNYI AHRSSNLRSL 

       130        140        150        160        170        180 
RLIRCSQITD DGFVEAVVKL PLEELELSYC SFSVESLRVV GQCCLNMKTL KLNKHPQKEN 

       190        200        210        220        230        240 
DDDALAIAET MPKLRHLQLC GNGLSDTGLN AILDNCSNLE HLDLRRCFNV NLVGDLQKRC 

       250        260        270        280        290        300 
FESVKVVRHP NDSIHDIDIG SSEDEDPYDF SDIDLMSGDD DFEGYYDFSG ASDFSDYDQF 


DF 

References and documentation are available.

Number of amino acids: 302

Molecular weight: 34158.33

Theoretical pI: 4.56

Amino acid composition: 
Ala (A)  14	  4.6%
Arg (R)  17	  5.6%
Asn (N)  16	  5.3%
Asp (D)  34	 11.3%
Cys (C)  11	  3.6%
Gln (Q)   8	  2.6%
Glu (E)  19	  6.3%
Gly (G)  17	  5.6%
His (H)   9	  3.0%
Ile (I)  16	  5.3%
Leu (L)  35	 11.6%
Lys (K)  11	  3.6%
Met (M)   9	  3.0%
Phe (F)  11	  3.6%
Pro (P)  10	  3.3%
Ser (S)  28	  9.3%
Thr (T)   8	  2.6%
Trp (W)   4	  1.3%
Tyr (Y)   8	  2.6%
Val (V)  17	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1483
Hydrogen    H	      2312
Nitrogen    N	       410
Oxygen      O	       477
Sulfur      S	        20

Formula: C1483H2312N410O477S20
Total number of atoms: 4702

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34545
Abs 0.1% (=1 g/l)   1.011, assuming all pairs of Cys residues form cystines


Ext. coefficient    33920
Abs 0.1% (=1 g/l)   0.993, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.25
This classifies the protein as unstable.



Aliphatic index: 86.82

Grand average of hydropathicity (GRAVY): -0.349