ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSSSSRNW LYDVFLSFRG GDVRVTFRSH FLKEFDRKLI TAFRDNEIER SHSLWPDLEQ 

        70         80         90        100        110        120 
AIKESRIAVV VFSKNYASSS WCLNELLEIV NCNDKIIIPV FYGVDPSQVR YQIGEFGKIF 

       130        140        150        160        170        180 
EKTCKRQTEE VKNQWKKALT HVANMLGFDS SKWDDEAKMI EEIANDVLRK LLLTTSKDFE 

       190        200        210        220        230        240 
DFVGLEDHIA NMSALLDLES KEVKMVGIWG SSGIGKTTIA RALFNNLFRH FQVRKFIDRS 

       250        260        270        280        290        300 
FAYKSREIHS SANPDDHNMK LHLQESFLSE ILRMPNIKID HLGVLGERLQ HQKVLIIIDD 

       310        320        330        340        350        360 
VDDQVILDSL VGKTQWFGNG SRIIVVTNNK HFLTAHGIDR MYEVSLPTEE HALAMLCQSA 

       370        380        390        400        410        420 
FKKKSPPEGF EMLVVQVARY AGSLPLVLKV LGSYLSGKDK EYWIDMLPRL QNGLNDKIER 

       430        440        450        460        470        480 
ILRISYDGLE SEDQAIFRHI ACIFNHMEVT TIKSLLANSI YGANVGLQNL VDKSIIHVRW 

       490        500        510        520        530        540 
GHVEMHPLLQ EMGRKIVRTQ SIGKPRKREF LVDPNDICDV LSEGIDTQKV LGISLETSKI 

       550        560        570        580        590        600 
DELCVHESAF KRMRNLRFLK IGTDIFGEEN RLHLPESFDY LPPTLKLLCW SEFPMRCMPS 

       610        620        630        640        650        660 
NFCPKNLVTL KMTNSKLHKL WEGAVPLTCL KEMDLDGSVN LKEIPDLSMA TNLETLNFEN 

       670        680        690        700        710        720 
CKSLVELPSF IQNLNKLLKL NMAFCNSLET LPTGFNLKSL NRIDFTKCSK LRTFPDFSTN 

       730        740        750        760        770        780 
ISDLYLTGTN IEELPSNLHL ENLIDLRISK KEIDGKQWEG VMKPLKPLLA MLSPTLTSLQ 

       790        800        810        820        830        840 
LQNIPNLVEL PCSFQNLIQL EVLDITNCRN LETLPTGINL QSLDSLSFKG CSRLRSFPEI 

       850        860        870        880        890        900 
STNISSLNLE ETGIEEVPWW IDKFSNLGLL SMDRCSRLKC VSLHISKLKR LGKVDFKDCG 

       910        920        930        940        950        960 
ALTIVDLCGC PIGMEMEANN IDTVSKVKLD FRDCFNLDPE TVLHQESIIF KYMLFPGKEE 

       970        980        990       1000       1010       1020 
MPSYFTYRTT GSSSLTIPLL HLPLSQPFFR FRVGALVTNV KHGKNIKVKC EFKDRFGNSF 

      1030       1040       1050       1060       1070       1080 
HVGSDDFYVY LLFTKSQKGS QMLTILDCCI PLNEGNASLA QGNYYDHVDI NIHISSGGWR 

      1090       1100       1110       1120       1130       1140 
STFELKEWGI RLLEEDSSSA ENGLGNPNST LPHVSEAEEG NMGYYTPLQE GLVNEIEHSE 

      1150       1160       1170       1180       1190       1200 
ESGDINVGTK RSKKRMRPKK TIWGIHRFKN LLRSRKRMRV SIKNERPLKK SRTRPASIPR 


TGRNT 

References and documentation are available.

Number of amino acids: 1205

Molecular weight: 137472.57

Theoretical pI: 7.71

Amino acid composition: 
Ala (A)  38	  3.2%
Arg (R)  64	  5.3%
Asn (N)  72	  6.0%
Asp (D)  65	  5.4%
Cys (C)  26	  2.2%
Gln (Q)  32	  2.7%
Glu (E)  85	  7.1%
Gly (G)  67	  5.6%
His (H)  32	  2.7%
Ile (I)  83	  6.9%
Leu (L) 149	 12.4%
Lys (K)  88	  7.3%
Met (M)  32	  2.7%
Phe (F)  59	  4.9%
Pro (P)  48	  4.0%
Ser (S) 104	  8.6%
Thr (T)  57	  4.7%
Trp (W)  17	  1.4%
Tyr (Y)  22	  1.8%
Val (V)  65	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 150
Total number of positively charged residues (Arg + Lys): 152

Atomic composition:

Carbon      C	      6136
Hydrogen    H	      9758
Nitrogen    N	      1670
Oxygen      O	      1793
Sulfur      S	        58

Formula: C6136H9758N1670O1793S58
Total number of atoms: 19415

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   127905
Abs 0.1% (=1 g/l)   0.930, assuming all pairs of Cys residues form cystines


Ext. coefficient   126280
Abs 0.1% (=1 g/l)   0.919, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.34
This classifies the protein as unstable.



Aliphatic index: 93.88

Grand average of hydropathicity (GRAVY): -0.266