ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASSTALICD TEAWKDLKGH VEDIKKTHLR DLMSDANRCQ SMMMEFDGLL LDYSRQRATV 

        70         80         90        100        110        120 
ETMDKLLNLA KASQLTEKIS RMFNGEHINS TENRSVLHVA LRAPKDAVIK ADGMNVVPEV 

       130        140        150        160        170        180 
WNVLDKIKEF SDKIRSGSWV GATGKPLKDV IAIGIGGSFL GPLFVHTALQ TDPEALESAK 

       190        200        210        220        230        240 
GRQLRFLANI DPVDVARNIS GLNPETTLVV VVSKTFTTAE TMLNARTLRE WITAALGASA 

       250        260        270        280        290        300 
VAKHMVAVST NLALVEKFGI DPNNAFAFWD WVGGRYSVCS AVGVLPLSLQ YGFSMVEKFL 

       310        320        330        340        350        360 
KGASSIDQHF QSTPFEKNIP VLLGLLSVWN VSFLGYPARA ILPYSQALEK FAPHIQQVSM 

       370        380        390        400        410        420 
ESNGKGVSID GLPLPFETGE IDFGEPGTNG QHSFYQLIHQ GRVIPCDFIG IVKSQQPVYL 

       430        440        450        460        470        480 
KGEVVSNHDE LMSNFFAQPD ALAYGKTPEQ LQKENVSENL IPHKTFSGNR PSLSLLLPEL 

       490        500        510        520        530        540 
TAYNVGQLLA IYEHRVAVQG FVWGINSFDQ WGVELGKVLA TQVRKQLHSS RTQGTAPEGF 

       550        560 
NYSTTTLLKR YLETSSEPQM 

References and documentation are available.

Number of amino acids: 560

Molecular weight: 61717.59

Theoretical pI: 6.19

Amino acid composition: 
Ala (A)  43	  7.7%
Arg (R)  21	  3.8%
Asn (N)  26	  4.6%
Asp (D)  25	  4.5%
Cys (C)   4	  0.7%
Gln (Q)  26	  4.6%
Glu (E)  33	  5.9%
Gly (G)  41	  7.3%
His (H)  14	  2.5%
Ile (I)  27	  4.8%
Leu (L)  60	 10.7%
Lys (K)  31	  5.5%
Met (M)  14	  2.5%
Phe (F)  26	  4.6%
Pro (P)  26	  4.6%
Ser (S)  45	  8.0%
Thr (T)  32	  5.7%
Trp (W)   9	  1.6%
Tyr (Y)  12	  2.1%
Val (V)  45	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2769
Hydrogen    H	      4353
Nitrogen    N	       743
Oxygen      O	       818
Sulfur      S	        18

Formula: C2769H4353N743O818S18
Total number of atoms: 8701

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67630
Abs 0.1% (=1 g/l)   1.096, assuming all pairs of Cys residues form cystines


Ext. coefficient    67380
Abs 0.1% (=1 g/l)   1.092, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.53
This classifies the protein as stable.



Aliphatic index: 91.57

Grand average of hydropathicity (GRAVY): -0.107