ProtParam
User-provided sequence:
10 20 30 40 50 60
MASTDFSKIE TLKQLNASLA DKSYIEGTAV SQADVTVFKA FQSAYPEFSR WFNHIASKAD
70 80 90 100 110 120
EFDSFPAASA AAAEEEEDDD VDLFGSDDEE ADAEAEKLKA ERIAAYNAKK AAKPAKPAAK
130 140 150 160 170 180
SIVTLDVKPW DDETNLEEMV ANVKAIEMEG LTWGAHQFIP IGFGIKKLQI NCVVEDDKVS
190 200
LDDLQQSIEE DEDHVQSTDI AAMQKL
References and
documentation are available.
Number of amino acids: 206
Molecular weight: 22627.04
Theoretical pI: 4.30
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 997
Hydrogen H 1546
Nitrogen N 254
Oxygen O 336
Sulfur S 5
Formula: C
997H
1546N
254O
336S
5
Total number of atoms: 3138
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20970
Abs 0.1% (=1 g/l) 0.927, assuming all pairs of Cys residues form cystines
Ext. coefficient 20970
Abs 0.1% (=1 g/l) 0.927, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.13
This classifies the protein as unstable.
Aliphatic index: 77.86
Grand average of hydropathicity (GRAVY): -0.417