ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN KNKENNTFTW 

        70         80         90        100        110        120 
NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF TTSEATANDR QINNVHLIPN 

       130        140        150        160        170        180 
EIKGASETPL QKTVNLKNIM KVSDPYVPTR NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY 

       190        200        210        220        230        240 
EDNYNPKMQW SLQDNSAAIN NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE 

       250        260        270        280        290        300 
PLLSLDVTPI SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE 

       310        320        330        340        350        360 
DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS TATTGKRKPA 

       370        380        390        400        410        420 
KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN YTKKHLSSHS STNSNSKPST 

       430        440        450        460        470        480 
ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC 

       490        500        510        520        530 
IKILGSEGYQ KTFSRLDALT RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G 

References and documentation are available.

Number of amino acids: 531

Molecular weight: 60152.70

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  25	  4.7%
Arg (R)  21	  4.0%
Asn (N)  51	  9.6%
Asp (D)  40	  7.5%
Cys (C)   6	  1.1%
Gln (Q)  22	  4.1%
Glu (E)  39	  7.3%
Gly (G)  15	  2.8%
His (H)  18	  3.4%
Ile (I)  30	  5.6%
Leu (L)  35	  6.6%
Lys (K)  34	  6.4%
Met (M)   7	  1.3%
Phe (F)  21	  4.0%
Pro (P)  22	  4.1%
Ser (S)  65	 12.2%
Thr (T)  41	  7.7%
Trp (W)   2	  0.4%
Tyr (Y)  18	  3.4%
Val (V)  19	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2592
Hydrogen    H	      4029
Nitrogen    N	       739
Oxygen      O	       887
Sulfur      S	        13

Formula: C2592H4029N739O887S13
Total number of atoms: 8260

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38195
Abs 0.1% (=1 g/l)   0.635, assuming all pairs of Cys residues form cystines


Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.629, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.17
This classifies the protein as unstable.



Aliphatic index: 62.82

Grand average of hydropathicity (GRAVY): -0.911