ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASTLRNIHD QNVIASEDTV YRYLCPVKKT GSIIGKGGEI AKQIRSETKS NMRINEALPG 

        70         80         90        100        110        120 
CEERVVTMYS TNEELNHFGD DGELVCPALD ALFKVHDMVV ADADQDDGTD DDNDLGEKQT 

       130        140        150        160        170        180 
VTVRMLVPSD QIGCVIGKGG QVIQNLRNDT NAQIRVIKDH LPACALTLSH DELLLIIGEP 

       190        200        210        220        230        240 
LVVREALYQV ASLLHDNPSR FQHLLLSSSS SSMHQPGAML MSAALTSSHR NYAVRRDIAD 

       250        260        270        280        290        300 
AREFCVCFIC PAENVGGVIG KGGGFINQIR QETGATIRVN TSETDDDDCI IFISSKEFYE 

       310        320        330        340        350        360 
DQSPAVNAAI RLQQRCSEKV GKDANDLAIS TRLLVSSSQI GCLIGKGGAV ISEMRSVTRA 

       370        380        390        400        410        420 
NIRILQKEDV PKIAREDEEM VQITGSPDAA MKALTQVILR LRANSFDMDH GLVLLPTSFP 

       430        440        450 
YIPQVTESSS KSKYAKRDDH SKLNSNSKRR NHVS 

References and documentation are available.

Number of amino acids: 454

Molecular weight: 49769.37

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  34	  7.5%
Arg (R)  28	  6.2%
Asn (N)  22	  4.8%
Asp (D)  35	  7.7%
Cys (C)  11	  2.4%
Gln (Q)  21	  4.6%
Glu (E)  26	  5.7%
Gly (G)  29	  6.4%
His (H)  12	  2.6%
Ile (I)  34	  7.5%
Leu (L)  38	  8.4%
Lys (K)  22	  4.8%
Met (M)  12	  2.6%
Phe (F)  10	  2.2%
Pro (P)  15	  3.3%
Ser (S)  41	  9.0%
Thr (T)  22	  4.8%
Trp (W)   0	  0.0%
Tyr (Y)   8	  1.8%
Val (V)  34	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2138
Hydrogen    H	      3487
Nitrogen    N	       627
Oxygen      O	       691
Sulfur      S	        23

Formula: C2138H3487N627O691S23
Total number of atoms: 6966

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12545
Abs 0.1% (=1 g/l)   0.252, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.240, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.23
This classifies the protein as unstable.



Aliphatic index: 91.06

Grand average of hydropathicity (GRAVY): -0.283