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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASTTCAREV HYRGVRKRPW GRYAAEIRDP WKKTRVWLGT FDTPEEAALA YDGAARFLRG

70 80 90 100 110 120
IKAKTNFPSP LSLDLNHLPS APSAATAAAN NQPHQHQQLW FAAPPPVPPS SDHHHQHHRI

130 140 150 160 170 180
FLRTGVLNDK TSDYSSTEAP LYFTSSPNTA TSSPGYQVVG FPMMNSSPSP VTVRRGLAID


LNEPPPLWL


References and documentation are available.

Number of amino acids: 189 Molecular weight: 20946.57 Theoretical pI: 9.56
Amino acid composition: 
Ala (A) 21 11.1% Arg (R) 13 6.9% Asn (N) 8 4.2% Asp (D) 8 4.2% Cys (C) 1 0.5% Gln (Q) 6 3.2% Glu (E) 6 3.2% Gly (G) 9 4.8% His (H) 9 4.8% Ile (I) 4 2.1% Leu (L) 15 7.9% Lys (K) 6 3.2% Met (M) 3 1.6% Phe (F) 7 3.7% Pro (P) 22 11.6% Ser (S) 17 9.0% Thr (T) 14 7.4% Trp (W) 5 2.6% Tyr (Y) 6 3.2% Val (V) 9 4.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 939 Hydrogen H 1429 Nitrogen N 271 Oxygen O 269 Sulfur S 4 Formula: C939H1429N271O269S4 Total number of atoms: 2912 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 36440 Abs 0.1% (=1 g/l) 1.740, assuming all pairs of Cys residues form cystines Ext. coefficient 36440 Abs 0.1% (=1 g/l) 1.740, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 60.46 This classifies the protein as unstable. Aliphatic index: 64.13 Grand average of hydropathicity (GRAVY): -0.554