ProtParam
User-provided sequence:
10 20 30 40 50 60
MASVASSTTL ISSPSSRVFP AKSSLSSPSV SFLRTLSSPS ASASLRSGFA RRSSLSSTSR
70 80 90 100 110 120
RSFAVKAQAD DLPLVGNKAP DFEAEAVFDQ EFIKVKLSDY IGKKYVILFF YPLDFTFVCP
130 140 150 160 170 180
TEITAFSDRH SEFEKLNTEV LGVSVDSVFS HLAWVQTDRK SGGLGDLNYP LISDVTKSIS
190 200 210 220 230 240
KSFGVLIHDQ GIALRGLFII DKEGVIQHST INNLGIGRSV DETMRTLQAL QYIQENPDEV
250 260
CPAGWKPGEK SMKPDPKLSK EYFSAI
References and
documentation are available.
Number of amino acids: 266
Molecular weight: 29092.02
Theoretical pI: 6.91
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1305
Hydrogen H 2053
Nitrogen N 343
Oxygen O 399
Sulfur S 5
Formula: C
1305H
2053N
343O
399S
5
Total number of atoms: 4105
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20065
Abs 0.1% (=1 g/l) 0.690, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.685, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.32
This classifies the protein as unstable.
Aliphatic index: 86.13
Grand average of hydropathicity (GRAVY): -0.112