ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVEDATKV QEMLDALKAE YNALAHHSFA SKARGNDYES SMIQSQIQEI EAFRKTVDDM 

        70         80         90        100        110        120 
YEKQKSIRET YEKDINKLKR ELEELGVEAN TASYRNRGER SELAASNNQV THIDQEHPSQ 

       130        140        150        160        170        180 
TKSTSQPPSN HLPAFQQIPP IHQSAYPQNN VAEVLMPPIP PSVEASSGQN FNQGIASQNP 

       190        200        210        220        230        240 
AISTSNLPST TPLYIPPVNY GANQVSQQPN PQLPGVSNYY NPSATSKPAV NVQPPRIPTK 

       250        260        270        280        290        300 
ATPSAEPSMT ASANAGSISQ AGPDGEYQGR EQIAPVSDTE AARKTTSQSW YVTYNPACKR 

       310        320        330        340        350        360 
VFNINLVHTL EHPSVVCCVK FSNNGKYLAT GCNQAANVFD VQTGKKLFTL HEESPDPSRD 

       370        380        390        400        410        420 
LYVRTIAFSP DGKYLVTGTE DRQIKLWDLS TQKVRYVFSG HEQDIYSLDF SHNGRFIVSG 

       430        440        450        460        470        480 
SGDRTARLWD VETGQCILKL EIENGVTAIA ISPNDQFIAV GSLDQIIRVW SVSGTLVERL 

       490        500        510        520        530        540 
EGHKESVYSI AFSPDSSILL SGSLDKTIKV WELQATRSVG LSAIKPEGIC KATYTGHTDF 

       550        560        570        580        590        600 
VLSVAVSPDS RWGLSGSKDR SMQFWDLQTG QSYLTCQGHK NSVISVCFSP DGRQFASGSG 

       610 
DLRARIWSID PASP 

References and documentation are available.

Number of amino acids: 614

Molecular weight: 67330.98

Theoretical pI: 5.83

Amino acid composition: 
Ala (A)  48	  7.8%
Arg (R)  26	  4.2%
Asn (N)  32	  5.2%
Asp (D)  30	  4.9%
Cys (C)   8	  1.3%
Gln (Q)  39	  6.4%
Glu (E)  35	  5.7%
Gly (G)  36	  5.9%
His (H)  14	  2.3%
Ile (I)  35	  5.7%
Leu (L)  38	  6.2%
Lys (K)  29	  4.7%
Met (M)   7	  1.1%
Phe (F)  18	  2.9%
Pro (P)  40	  6.5%
Ser (S)  72	 11.7%
Thr (T)  37	  6.0%
Trp (W)   8	  1.3%
Tyr (Y)  21	  3.4%
Val (V)  41	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2953
Hydrogen    H	      4612
Nitrogen    N	       828
Oxygen      O	       946
Sulfur      S	        15

Formula: C2953H4612N828O946S15
Total number of atoms: 9354

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    75790
Abs 0.1% (=1 g/l)   1.126, assuming all pairs of Cys residues form cystines


Ext. coefficient    75290
Abs 0.1% (=1 g/l)   1.118, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.56
This classifies the protein as unstable.



Aliphatic index: 73.55

Grand average of hydropathicity (GRAVY): -0.494