ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVLGSGRG SGGLSSQLKC KSKRRRRRRS KRKDKVSILS TFLAPFKYLS PGTTNTEDED 

        70         80         90        100        110        120 
NLSTSSAEVK ENRNVSNLGT RPLPPGDWAR GGSTPSVKRK RPLEEGNGGH FCKLQLIWKK 

       130        140        150        160        170        180 
LSWSMTPKNA LVQLHELKPG LQYRMVSQTG PVHAPVFAVA VEVNGLTFEG TGPTKKKAKM 

       190        200        210        220        230        240 
RAAEMALKSF VQFPNAFQAH LAMGSSTSPC TDFTSDQADF PDTLFKEFEP SSRNEDFPGC 

       250        260        270        280        290        300 
CPVDTEFLSS AYRRGRLLYH TLDLMGQALP DRSRLAPGAL GERNPVVVLN ELRSGLRYVC 

       310        320        330        340        350        360 
LSETAEKPRV KSFVMAVCVD GRTFEGSGRS KKLAKGQAAQ AALQALFDIR LPGHIPSRSK 

       370        380        390        400        410        420 
SNLLPQDFAD SVSQLVTQKF RELTVGLTSV YARHKTLAGI VMTKGLDTKQ AQVIVLSSGT 

       430        440        450        460        470        480 
KCISGEHISD QGLVVNDCHA EIVARRAFLH FLYTQLELHL SKHQEDPERS IFIRVKEGGY 

       490        500        510        520        530        540 
RLRENILFHL YVSTSPCGDA RLNSPYEITI DLNSSKHIVR KFRGHLRTKI ESGEGTVPVR 

       550        560        570        580        590        600 
GPSAVQTWDG ILLGEQLVTM SCTDKIASWN VLGLQGALLC HFIEPVYLHS IIVGSLHHTG 

       610        620        630        640        650        660 
HLARVMSHRM EGIGQLPASY RQNRPLLSGV SNAEARQPGK SPHFSANWVV GSADLEIINA 

       670        680        690        700        710        720 
TTGKRSCGGS SRLCKHVFSA RWARLHGRLS TRIPGHGDTP SMYCEAKRGA HTYQSVKQQL 

       730        740 
FKAFQKAGLG TWVRKPPEQD QFLLSL 

References and documentation are available.

Number of amino acids: 746

Molecular weight: 82227.23

Theoretical pI: 9.79

Amino acid composition: 
Ala (A)  52	  7.0%
Arg (R)  55	  7.4%
Asn (N)  22	  2.9%
Asp (D)  27	  3.6%
Cys (C)  15	  2.0%
Gln (Q)  32	  4.3%
Glu (E)  37	  5.0%
Gly (G)  63	  8.4%
His (H)  26	  3.5%
Ile (I)  25	  3.4%
Leu (L)  79	 10.6%
Lys (K)  46	  6.2%
Met (M)  13	  1.7%
Phe (F)  30	  4.0%
Pro (P)  39	  5.2%
Ser (S)  72	  9.7%
Thr (T)  42	  5.6%
Trp (W)   8	  1.1%
Tyr (Y)  14	  1.9%
Val (V)  49	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      3627
Hydrogen    H	      5807
Nitrogen    N	      1071
Oxygen      O	      1057
Sulfur      S	        28

Formula: C3627H5807N1071O1057S28
Total number of atoms: 11590

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65735
Abs 0.1% (=1 g/l)   0.799, assuming all pairs of Cys residues form cystines


Ext. coefficient    64860
Abs 0.1% (=1 g/l)   0.789, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.20
This classifies the protein as unstable.



Aliphatic index: 80.39

Grand average of hydropathicity (GRAVY): -0.355