ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVLLPQLR TGWHVDQAIV TETKRLVVIR FGRKNDRQCM IMDELLSSIA ERVRNFAVIY 

        70         80         90        100        110        120 
LCDIDEVSDF DEMYELTDPM TVMFFYHNKH MMCDFGTGNN NKLNFIVDDK QEMIDILETI 

       130        140 
FRGARKNKGL VVSPYDYNHK RVS 

References and documentation are available.

Number of amino acids: 143

Molecular weight: 16776.37

Theoretical pI: 5.78

Amino acid composition: 
Ala (A)   5	  3.5%
Arg (R)  10	  7.0%
Asn (N)   9	  6.3%
Asp (D)  13	  9.1%
Cys (C)   3	  2.1%
Gln (Q)   4	  2.8%
Glu (E)   8	  5.6%
Gly (G)   6	  4.2%
His (H)   4	  2.8%
Ile (I)  10	  7.0%
Leu (L)  11	  7.7%
Lys (K)   8	  5.6%
Met (M)   9	  6.3%
Phe (F)   8	  5.6%
Pro (P)   3	  2.1%
Ser (S)   6	  4.2%
Thr (T)   7	  4.9%
Trp (W)   1	  0.7%
Tyr (Y)   5	  3.5%
Val (V)  13	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       741
Hydrogen    H	      1167
Nitrogen    N	       203
Oxygen      O	       217
Sulfur      S	        12

Formula: C741H1167N203O217S12
Total number of atoms: 2340

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   0.779, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.772, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.91
This classifies the protein as stable.



Aliphatic index: 87.13

Grand average of hydropathicity (GRAVY): -0.244