ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVPKRHTY GGNVVTDRDR HSLQRNNEIL HPIHKNQRKH ATFGPYIIGS TLGEGEFGKV 

        70         80         90        100        110        120 
KLGWTKASSS NEVPKQVAIK LIRRDTIKKD ADKEIKIYRE INALKHLTHP NIIYLEEVLQ 

       130        140        150        160        170        180 
NSKYIGIVLE FVSGGEFYKY IQRKRRLKES SACRLFAQLI SGVNYMHYKG LVHRDLKLEN 

       190        200        210        220        230        240 
LLLDKHENLV ITDFGFVNEF FEDNELMKTS CGSPCYAAPE LVVSTKAYEA RKADVWSCGV 

       250        260        270        280        290        300 
ILYAMLAGYL PWDDDHENPT GDDIARLYKY ITQTPLKFPE YITPIPRDLL RRILVPNPRR 

       310        320        330        340        350        360 
RINLQTIKRH VWLKPHEAFL SIQPNYWDEH LQKERPKPPN KGDVGRHSTY SSSASSYSKS 

       370        380        390        400        410        420 
RDRNSLIIES TLEQHRMSPQ LATSRPASPT FSTGSKVVLN DTKNDMKESN INGERTSASC 

       430        440        450        460        470        480 
RYTRDSKGNG QTQIEQVSAR HSSRGNKHTS VAGLVTIPGS PTTARTRNAP SSKLTEHVKD 

       490        500        510        520        530        540 
SSQTSFTQEE FHRIGNYHVP RSRPRPTSYY PGLSRNTADN SLADIPVNKL GSNGRLTDAK 

       550        560        570        580        590        600 
DPVPLNAIHD TNKATISNNS IMLLSEGPAA KTSPVDYHYA IGDLNHGDKP ITEVIDKINK 

       610        620        630        640        650        660 
DLTHKAAENG FPRESIDPES TSTILVTKEP TNSTDEDHVE SQLENVGHSS NKSDASSDKD 

       670        680        690        700        710        720 
SKKIYEKKRF SFMSLYSSLN GSRSTVESRT SKGNAPPVSS RNPSGQSNRS NIKITQQQPR 

       730        740        750        760        770        780 
NLSDRVPNPD KKINDNRIRD NAPSYAESEN PGRSVRASVM VSTLREENRS ELSNEGNNVE 

       790        800 
AQTSTARKVL NFFKRRSMRV 

References and documentation are available.

Number of amino acids: 800

Molecular weight: 90087.96

Theoretical pI: 9.50

Amino acid composition: 
Ala (A)  42	  5.2%
Arg (R)  59	  7.4%
Asn (N)  57	  7.1%
Asp (D)  43	  5.4%
Cys (C)   5	  0.6%
Gln (Q)  23	  2.9%
Glu (E)  49	  6.1%
Gly (G)  41	  5.1%
His (H)  27	  3.4%
Ile (I)  47	  5.9%
Leu (L)  57	  7.1%
Lys (K)  59	  7.4%
Met (M)  10	  1.2%
Phe (F)  19	  2.4%
Pro (P)  47	  5.9%
Ser (S)  88	 11.0%
Thr (T)  51	  6.4%
Trp (W)   5	  0.6%
Tyr (Y)  28	  3.5%
Val (V)  43	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 118

Atomic composition:

Carbon      C	      3923
Hydrogen    H	      6269
Nitrogen    N	      1175
Oxygen      O	      1232
Sulfur      S	        15

Formula: C3923H6269N1175O1232S15
Total number of atoms: 12614

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69470
Abs 0.1% (=1 g/l)   0.771, assuming all pairs of Cys residues form cystines


Ext. coefficient    69220
Abs 0.1% (=1 g/l)   0.768, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.71
This classifies the protein as unstable.



Aliphatic index: 71.54

Grand average of hydropathicity (GRAVY): -0.817