ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVPSLCDI LIPLEKNSRS DGDAESSNTV LIQLRKGHHE RMRSPYTIQK FYKFLKRAHC 

        70         80         90        100        110        120 
EENLEFFEKA HQFLQLKQNR SISEEKLLEV WNKSLYIKYI AVDSPKECNF SQDTREIFEK 

       130        140        150        160        170        180 
CFANNEVPAD VDVLCAISHV MGLLMDGYHR FVSSVNEKKY SATYAHNDSA TEQDLKNEST 

       190        200        210        220        230        240 
TSFSSLGVED ISEDRNSYLK KPDINGLSTI IQETSANTTN ESQCSDRTSR PSESSSSLNT 

       250        260        270        280        290        300 
TSSTYKNTST RNLQKPQNTG ILNSGKGLLQ KLNFVKKRKS FKQPSGVICS HYNSNVQNRL 


KGQNSHQQR 

References and documentation are available.

Number of amino acids: 309

Molecular weight: 35100.19

Theoretical pI: 8.70

Amino acid composition: 
Ala (A)  12	  3.9%
Arg (R)  15	  4.9%
Asn (N)  25	  8.1%
Asp (D)  14	  4.5%
Cys (C)   7	  2.3%
Gln (Q)  17	  5.5%
Glu (E)  22	  7.1%
Gly (G)  11	  3.6%
His (H)   9	  2.9%
Ile (I)  15	  4.9%
Leu (L)  26	  8.4%
Lys (K)  26	  8.4%
Met (M)   4	  1.3%
Phe (F)  12	  3.9%
Pro (P)   9	  2.9%
Ser (S)  42	 13.6%
Thr (T)  18	  5.8%
Trp (W)   1	  0.3%
Tyr (Y)  10	  3.2%
Val (V)  14	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1518
Hydrogen    H	      2415
Nitrogen    N	       441
Oxygen      O	       494
Sulfur      S	        11

Formula: C1518H2415N441O494S11
Total number of atoms: 4879

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20775
Abs 0.1% (=1 g/l)   0.592, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.581, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.47
This classifies the protein as unstable.



Aliphatic index: 68.77

Grand average of hydropathicity (GRAVY): -0.790