ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MASVVEYKGL KAGYYCGYCE SREGKASCGM WAHSMTVQDY QDLIDRGWRR SGKYVYKPVM 

        70         80         90        100        110        120 
NQTCCPQYTI RCHPLQFQPS KSHKKVLKKM LKFLAKGEIS KGNCEDEPMD STMEDAVDGD 

       130        140        150        160        170        180 
FALMNKLDIK CDLKTLSSDL KVSIESEEKE KEKSLKKEGS KDLIHPQAIE EKLGSGEPSH 

       190        200        210        220        230        240 
SIKVHVGPKP GKGADLSKPP CRKAKEMRKE RKRLKLMQQS SAGAFEGFQA QGQPVSLLPK 

       250        260        270        280        290        300 
AKSNQPKSLE DLIFESLPED ASHKLEVRVV RSSPPSPQFK ATFQESYQVY KRYQMIIHKD 

       310        320        330        340        350        360 
PPDKPTVSQF TRFLCNSPLE AEHPANGPEC GYGSFHQQYW LDGKIIAVGV IDILPYCVSS 

       370        380        390        400        410        420 
VYLYYDPDYS FLSLGVYSAL REIAFTRQLH EKTSQLSYYY MGFYIHSCPK MRYKGQYRPS 

       430        440        450        460        470        480 
DLLCPETYVW VPIEQCLPSL DNSKYCRFNQ DPQAEDEGRS KELDRLRVFH RRSAMPYGVY 

       490        500        510 
KNHQEDPSEE AVVLEYANLV GQKCSETMLL FRH 

References and documentation are available.

Number of amino acids: 513

Molecular weight: 58701.09

Theoretical pI: 8.47

Amino acid composition: 
Ala (A)  25	  4.9%
Arg (R)  24	  4.7%
Asn (N)  10	  1.9%
Asp (D)  27	  5.3%
Cys (C)  17	  3.3%
Gln (Q)  28	  5.5%
Glu (E)  39	  7.6%
Gly (G)  30	  5.8%
His (H)  15	  2.9%
Ile (I)  18	  3.5%
Leu (L)  43	  8.4%
Lys (K)  49	  9.6%
Met (M)  15	  2.9%
Phe (F)  17	  3.3%
Pro (P)  34	  6.6%
Ser (S)  48	  9.4%
Thr (T)  12	  2.3%
Trp (W)   4	  0.8%
Tyr (Y)  30	  5.8%
Val (V)  28	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      2607
Hydrogen    H	      4058
Nitrogen    N	       706
Oxygen      O	       774
Sulfur      S	        32

Formula: C2607H4058N706O774S32
Total number of atoms: 8177

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67700
Abs 0.1% (=1 g/l)   1.153, assuming all pairs of Cys residues form cystines


Ext. coefficient    66700
Abs 0.1% (=1 g/l)   1.136, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.40
This classifies the protein as unstable.



Aliphatic index: 67.08

Grand average of hydropathicity (GRAVY): -0.665