ProtParam
User-provided sequence:
10 20 30 40 50 60
MASWSAPSPS LVEYFEGQTS FQCGYCKSNF GSRSHGMWAH SMTVQDYQDL IDRGWRRSGK
70 80 90 100 110 120
YVYKPVMNQT CCPQYTIRCH PLQFQPSKSH KKVLKKMLKF LAKGEISKGN CEDEPMDSTM
130 140 150 160 170 180
EDAVDGDFAL MNKLDIKCDL KTLSSDLKVS IESEEKEKEK SLKKEGSKDL IHPQAIEEKL
190 200 210 220 230 240
GSGEPSHSIK VHVGPKPGKG ADLSKPPCRK AKEMRKERKR LKLMQQSSAG AFEGFQAQGQ
250 260 270 280 290 300
PVSLLPKAKS NQPKSLEDLI FESLPEDASH KLEVRLVPAS FEDPEFKSSF NQSLSLYTKY
310 320 330 340 350 360
QVAIHQDEPD ICDKSEFTRF LCNSPLEAEH PANGPECGYG SFHQQYWLDG KIIAVGVIDI
370 380 390 400 410 420
LPYCVSSVYL YYDPDYSFLS LGVYSALREI AFTRQLHEKT SQLSYYYMGF YIHSCPKMRY
430 440 450 460 470 480
KGQYRPSDLL CPETYVWVPI EQCLPSLDNS KYCRFNQDPQ AEDEGRSKEL DRLRVFHRRS
490 500 510 520
AMPYGVYKNH QEDPSEEAVV LEYANLVGQK CSETMLLFRH
References and
documentation are available.
Number of amino acids: 520
Molecular weight: 59422.53
Theoretical pI: 6.76
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2638
Hydrogen H 4077
Nitrogen N 709
Oxygen O 794
Sulfur S 31
Formula: C
2638H
4077N
709O
794S
31
Total number of atoms: 8249
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68730
Abs 0.1% (=1 g/l) 1.157, assuming all pairs of Cys residues form cystines
Ext. coefficient 67730
Abs 0.1% (=1 g/l) 1.140, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.20
This classifies the protein as unstable.
Aliphatic index: 66.75
Grand average of hydropathicity (GRAVY): -0.647