ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATEGVVPSA QDIAAEFVRQ YYHVLGQLPH EARRLYVDAS VVSRPDVTGT MMSFTSVEAI 

        70         80         90        100        110        120 
NKHILSCDFE NTKFEVLSVD SQNSLEDGIF IMVIGFMTGK DNQRRKFSQM FYLARQNTLV 

       130        140        150        160        170        180 
VLNDMLRYVD QEDSSTTETP CEPVTEIVRP ADGLKKAEKT ELKQKNVASV EKSVNAAVEK 

       190        200        210        220        230        240 
NAAPLDNGKM KQSEKAVITQ KVTEPDAAPQ PDGAKRSFAD IVGSMAKNAA PFQVKSPVQA 

       250        260        270        280        290        300 
PVQKPKYVGQ PRAAAAPQKP AYVSKSIKKN DQKVIEVPGT SIFVANLPLN AMPPQLFELF 

       310        320        330        340        350        360 
KDFGPIKENG IQVRSSRGNA NPVCFGFISF ETVASVQSVL QAAKNTPFML ADRKLRVKEK 

       370        380        390        400        410        420 
EVDYDGSKPS GKTKGGSNKT QNGSADSSKT ENGSADDSKT NGSAEDGEKE FKQVKSRRNR 


KKSEAAH 

References and documentation are available.

Number of amino acids: 427

Molecular weight: 46674.83

Theoretical pI: 9.08

Amino acid composition: 
Ala (A)  41	  9.6%
Arg (R)  18	  4.2%
Asn (N)  23	  5.4%
Asp (D)  24	  5.6%
Cys (C)   3	  0.7%
Gln (Q)  24	  5.6%
Glu (E)  28	  6.6%
Gly (G)  24	  5.6%
His (H)   4	  0.9%
Ile (I)  15	  3.5%
Leu (L)  20	  4.7%
Lys (K)  42	  9.8%
Met (M)  11	  2.6%
Phe (F)  19	  4.4%
Pro (P)  25	  5.9%
Ser (S)  37	  8.7%
Thr (T)  21	  4.9%
Trp (W)   0	  0.0%
Tyr (Y)   8	  1.9%
Val (V)  40	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2040
Hydrogen    H	      3282
Nitrogen    N	       578
Oxygen      O	       645
Sulfur      S	        14

Formula: C2040H3282N578O645S14
Total number of atoms: 6559

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12045
Abs 0.1% (=1 g/l)   0.258, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.255, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.34
This classifies the protein as unstable.



Aliphatic index: 68.74

Grand average of hydropathicity (GRAVY): -0.566