ProtParam
User-provided sequence:
10 20 30 40 50 60
MATEQEFTRA SRFSRDSSSV GYYSEEDNTE EEDEEEEEME EIEEEEEEEE EEDPRIGLTC
70 80 90 100 110 120
GGRRNGSSNN NKWMMLGRIL DPRSKWVREW NKVFLLVCAT GLFVDPLFLY TLSVSDTCMC
130 140 150 160 170 180
LLVDGWLALT VTALRSMTDL LHLWNIWIQF KIARRWPYPG GDSDGDTNKG GGTRGSTRVA
190 200 210 220 230 240
PPYVKKNGFF FDLFVILPLP QVVLWVVIPS LLKRGSVTLV VSVLLVTFLF QYLPKIYHSI
250 260 270 280 290 300
RHLRRNATLS GYIFGTVWWG IALNMIAYFV AAHAAGACWY LLGVQRSAKC LKEQCENTIG
310 320 330 340 350 360
CDLRMLSCKE PVYYGTTVMV LDRARLAWAQ NHQARSVCLD INTNYTYGAY QWTIQLVSSE
370 380 390 400 410 420
SRLEKILFPI FWGLMTLSTF GNLESTTEWS EVVFNIIVLT SGLLLVTMLI GNIKVFLHAT
430 440 450 460 470 480
TSKKQAMHLK MRNIEWWMKK RHLPIGFRQR VRNYERQRWA AMRGVDECEM VQNLPEGLRR
490 500 510 520 530 540
DIKYHLCLDL VRQVPLFQHM DDLVLENICD RVKSLIFTKG ETIQKEGDAV QRMLFVVRGH
550 560 570 580 590 600
LQSSQLLRDG VKSCCMLGPG NFSGDELLSW CLRRPFVERL PPSSSTLVTL ETTEAFGLDA
610 620 630 640 650 660
EDVKYVTQHF RYTFVNEKVK RSARYYSPGW RTWAAVAVQL AWRRYKHRLT LTSLSFIRPR
670 680 690
RPLSRCASLG EDKLRLYAAI LTSPKPNPDD FDDY
References and
documentation are available.
Number of amino acids: 694
Molecular weight: 80081.35
Theoretical pI: 8.20
Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 84
Atomic composition:
Carbon C 3604
Hydrogen H 5615
Nitrogen N 981
Oxygen O 1015
Sulfur S 36
Formula: C
3604H
5615N
981O
1015S
36
Total number of atoms: 11251
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 157760
Abs 0.1% (=1 g/l) 1.970, assuming all pairs of Cys residues form cystines
Ext. coefficient 156760
Abs 0.1% (=1 g/l) 1.958, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.54
This classifies the protein as unstable.
Aliphatic index: 90.42
Grand average of hydropathicity (GRAVY): -0.182