ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ 

        70         80         90        100        110        120 
EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD 

       130        140        150        160        170        180 
DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP 

       190        200        210        220        230        240 
VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK 

       250        260        270        280        290        300 
HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN 

       310 
VLQSSHLEDY PFDAEY 

References and documentation are available.

Number of amino acids: 316

Molecular weight: 36020.56

Theoretical pI: 7.12

Amino acid composition: 
Ala (A)  24	  7.6%
Arg (R)  10	  3.2%
Asn (N)  10	  3.2%
Asp (D)  18	  5.7%
Cys (C)   4	  1.3%
Gln (Q)  12	  3.8%
Glu (E)  25	  7.9%
Gly (G)  15	  4.7%
His (H)   9	  2.8%
Ile (I)  20	  6.3%
Leu (L)  29	  9.2%
Lys (K)  33	 10.4%
Met (M)   4	  1.3%
Phe (F)  15	  4.7%
Pro (P)  18	  5.7%
Ser (S)  16	  5.1%
Thr (T)  13	  4.1%
Trp (W)   6	  1.9%
Tyr (Y)  11	  3.5%
Val (V)  24	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1647
Hydrogen    H	      2569
Nitrogen    N	       425
Oxygen      O	       465
Sulfur      S	         8

Formula: C1647H2569N425O465S8
Total number of atoms: 5114

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49640
Abs 0.1% (=1 g/l)   1.378, assuming all pairs of Cys residues form cystines


Ext. coefficient    49390
Abs 0.1% (=1 g/l)   1.371, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.15
This classifies the protein as stable.



Aliphatic index: 90.09

Grand average of hydropathicity (GRAVY): -0.325