ProtParam
User-provided sequence:
10 20 30 40 50 60
MATIASEYSS EASNTPIEHQ FNPYGDNGGT ILGIAGEDFA VLAGDTRNIT DYSINSRYEP
70 80 90 100 110 120
KVFDCGDNIV MSANGFAADG DALVKRFKNS VKWYHFDHND KKLSINSAAR NIQHLLYGKR
130 140 150 160 170 180
FFPYYVHTII AGLDEDGKGA VYSFDPVGSY EREQCRAGGA AASLIMPFLD NQVNFKNQYE
190 200 210 220 230 240
PGTNGKVKKP LKYLSVEEVI KLVRDSFTSA TERHIQVGDG LEILIVTKDG VRKEFYELKR
D
References and
documentation are available.
Number of amino acids: 241
Molecular weight: 26871.09
Theoretical pI: 5.71
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1200
Hydrogen H 1843
Nitrogen N 325
Oxygen O 368
Sulfur S 5
Formula: C
1200H
1843N
325O
368S
5
Total number of atoms: 3741
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24995
Abs 0.1% (=1 g/l) 0.930, assuming all pairs of Cys residues form cystines
Ext. coefficient 24870
Abs 0.1% (=1 g/l) 0.926, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.28
This classifies the protein as stable.
Aliphatic index: 77.30
Grand average of hydropathicity (GRAVY): -0.437