ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATIEDIKET ALIPFQKHRQ LSMHEAEVIT LEIIGLLCDS ECKDEKTLKY LGRFLTPDMY 

        70         80         90        100        110        120 
QDLVDERNLN KRCGYPLCGK SPERIRDPFS MNDTTKKFLL ENNPYAYLSH YCSKFHFRCS 

       130        140        150        160        170        180 
QFYQVQLSDE ALFARTGVHL FEDPEQDKHD IDFKVTLFEE LLREKASEED IKSLISGLKK 

       190        200        210        220 
LGLNPDSGTT EKDDTELEDD LSKWLAQIKI VENDNPSILG DFTRED 

References and documentation are available.

Number of amino acids: 226

Molecular weight: 26239.63

Theoretical pI: 4.81

Amino acid composition: 
Ala (A)   8	  3.5%
Arg (R)  10	  4.4%
Asn (N)   8	  3.5%
Asp (D)  22	  9.7%
Cys (C)   6	  2.7%
Gln (Q)   8	  3.5%
Glu (E)  23	 10.2%
Gly (G)   9	  4.0%
His (H)   6	  2.7%
Ile (I)  13	  5.8%
Leu (L)  28	 12.4%
Lys (K)  19	  8.4%
Met (M)   4	  1.8%
Phe (F)  12	  5.3%
Pro (P)   9	  4.0%
Ser (S)  14	  6.2%
Thr (T)  13	  5.8%
Trp (W)   1	  0.4%
Tyr (Y)   7	  3.1%
Val (V)   6	  2.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1162
Hydrogen    H	      1818
Nitrogen    N	       304
Oxygen      O	       367
Sulfur      S	        10

Formula: C1162H1818N304O367S10
Total number of atoms: 3661

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16305
Abs 0.1% (=1 g/l)   0.621, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.607, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.24
This classifies the protein as unstable.



Aliphatic index: 81.99

Grand average of hydropathicity (GRAVY): -0.617