ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATLADIGLA AAINILSALI FLLLFAILRI QPFNDRVYFP KWYLKGVRSS PVNSGAFVSK 

        70         80         90        100        110        120 
IMNLDFRSYV RFLNWMPDAL KMPEPELIDH AGLDSAVYLR IYLIGLKIFG PIALLSWSIL 

       130        140        150        160        170        180 
VPVNWTSDGL QLAKLRNVTS SNIDKLSISN VERGSDRFWA HLVMAYAFTF WTCYVLMKEY 

       190        200        210        220        230        240 
EKIAAMRLSF LQSEKRRADQ FTVLVRNVPP DSDESISENV QHFFLVNHPD HYLTHQVVYN 

       250        260        270        280        290        300 
ANELAKLVED KKKMQNWLDY YQLKYTRNKE QRPRVKMGFL GLWGKKVDAM DHYTAEIEKL 

       310        320        330        340        350        360 
SEQIMEERKR IKKDDKSVMQ AAFVSFKTRW GAAVCAQTQQ TKNPTEWLTE WAPEAREMYW 

       370        380        390        400        410        420 
PNLAMPYVSL TVRRFVMHIA FFFLTFFFII PIAFVQSLAS IEGIEKSAPF LSPIVKNKLM 

       430        440        450        460        470        480 
KSLIQGFLPG IVLKLFLIFL PTILMIMSKF EGFISISSLE RRAAFRYYIF NLVNVFLGSV 

       490        500        510        520        530        540 
ITGSAFEQLD SFLKQSANDI PRTVGVAIPI KATFFITYIM VDGWAGVAGE IFRLKPLVIF 

       550        560        570        580        590        600 
HLKNFFFVKT EKDREEAMDP GQIDFYATEP RIQLYFLLGL VYAPVTPVLL PFIIFFFGFA 

       610        620        630        640        650        660 
YLVFRHQIIN VYNQKYESAG AFWPDVHGRI ISALIISQIL LLGLMSTKGK VQSTPFLLVL 

       670        680        690        700        710        720 
AILTFGFHRF CKGRYESAFV INPLQEAMIK DTLERAREPN LNLKGFLQNA YVHPVFKDEE 

       730        740        750        760        770 
DSDEEGLIED SDDEDCVVVQ TKRQRSRRTT VASSNASRGS SQSTPFNQLD LGKGKPET 

References and documentation are available.

Number of amino acids: 778

Molecular weight: 89309.12

Theoretical pI: 9.07

Amino acid composition: 
Ala (A)  57	  7.3%
Arg (R)  40	  5.1%
Asn (N)  31	  4.0%
Asp (D)  35	  4.5%
Cys (C)   4	  0.5%
Gln (Q)  30	  3.9%
Glu (E)  42	  5.4%
Gly (G)  36	  4.6%
His (H)  13	  1.7%
Ile (I)  58	  7.5%
Leu (L)  84	 10.8%
Lys (K)  48	  6.2%
Met (M)  22	  2.8%
Phe (F)  61	  7.8%
Pro (P)  36	  4.6%
Ser (S)  52	  6.7%
Thr (T)  33	  4.2%
Trp (W)  14	  1.8%
Tyr (Y)  27	  3.5%
Val (V)  55	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 88

Atomic composition:

Carbon      C	      4136
Hydrogen    H	      6387
Nitrogen    N	      1047
Oxygen      O	      1106
Sulfur      S	        26

Formula: C4136H6387N1047O1106S26
Total number of atoms: 12702

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117480
Abs 0.1% (=1 g/l)   1.315, assuming all pairs of Cys residues form cystines


Ext. coefficient   117230
Abs 0.1% (=1 g/l)   1.313, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.65
This classifies the protein as unstable.



Aliphatic index: 99.01

Grand average of hydropathicity (GRAVY): 0.077