ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATLEITDIA LVQPSHQPLS NDQTLSLSHL DNDNNLHVSF RYLRVYSSSS STVAGESPSA 

        70         80         90        100        110        120 
VVSASLATAL VHYYPLAGSL RRSASDNRFE LLCSAGQSVP LVNATVNCTL ESVGYLDGPD 

       130        140        150        160        170        180 
PGFVERLVPD PTREEGMVNP CILQVTMFQC GGWVLGASIH HAICDGLGAS LFFNAMAELA 

       190        200        210        220        230        240 
RGATKISIEP VWDRERLLGP REKPWVGAPV RDFLSLDKDF DPYGQAIGDV KRDCFFVTDD 

       250        260        270        280        290        300 
SLDQLKAQLL EKSGLNFTTF EALGAYIWRA KVRAAKTEEK ENVKFVYSIN IRRLMNPPLP 

       310        320        330        340        350        360 
KGYWGNGCVP MYAQIKAGEL IEQPIWKTAE LIKQSKSNTS DEYVRSFIDF QELHHKDGIN 

       370        380        390        400        410        420 
AGTGVTGFTD WRYLGHSTID FGWGGPVTVL PLSNKLLGSM EPCFFLPYST DAAAGSKKDS 

       430        440        450 
GFKVLVNLRE SAMPEFKEAM DKFHKGEFAL S 

References and documentation are available.

Number of amino acids: 451

Molecular weight: 49679.45

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  36	  8.0%
Arg (R)  20	  4.4%
Asn (N)  18	  4.0%
Asp (D)  27	  6.0%
Cys (C)   8	  1.8%
Gln (Q)  13	  2.9%
Glu (E)  26	  5.8%
Gly (G)  35	  7.8%
His (H)  10	  2.2%
Ile (I)  18	  4.0%
Leu (L)  47	 10.4%
Lys (K)  23	  5.1%
Met (M)   9	  2.0%
Phe (F)  23	  5.1%
Pro (P)  25	  5.5%
Ser (S)  39	  8.6%
Thr (T)  22	  4.9%
Trp (W)   8	  1.8%
Tyr (Y)  13	  2.9%
Val (V)  31	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      2227
Hydrogen    H	      3445
Nitrogen    N	       593
Oxygen      O	       663
Sulfur      S	        17

Formula: C2227H3445N593O663S17
Total number of atoms: 6945

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63870
Abs 0.1% (=1 g/l)   1.286, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   1.276, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.31
This classifies the protein as stable.



Aliphatic index: 84.12

Grand average of hydropathicity (GRAVY): -0.165